中国食品添加剂Issue(3)：164-171,8.
超高效液相色谱-四极杆/静电场轨道阱高分辨质谱快筛查椒粉中10种苏丹类色素
Rapid screening of 10 Sudan dyes in paprika by ultra high performance liquid chromatography-quadrupole/electrostatic field obitrap high-resolution mass spectrometry
摘要
Abstract
High performance liquid chromatography-quadrupole/electrostatic field obitrap high-resolution mass spectrometry was used to the determination of 10 Sudan dyes,i.e.,Sudan Orange G、Sudan Orange、Sudan Red G、Sudan Red 7B、Sudan Blue 2、Sudan Black B、Sudan Red Ⅰ、Sudan Red 2、Sudan Red 3 and Sudan Red 4 in Paprika.The samples were extracted by n-hexane,concentrated to dryness in nitrogen at 40℃.The residue was dissolved with 1mL acetonitrile,ZORBAX Eclipse Plus C18 (3.0 mm × 100 mm,1.8 μm) was used as chromatography colomn to separat the Sudan dyes with a gradient elution of acetonitrile and 0.1％ formic acid solution as the mobile phase.The retention time,accurate mass of parent ion and relative isotope abundance were used for fast screening in full scan mode,while accurate mass of fragment ions were used for comfirmation in two-stage threshold-triggered full mass scan mode.Under the optimal conditions,good linearities (r2 ＞ 0.99) were obtained in their respective linear ranges.The limit of detections (LODs) for 10 Sudan dyes were ≤ 25.0 μg/kg.The recoveries were in the range of 81.6％ ～ 109.3％ and the relative standard deviations (RSDs) were 5.3％ ～ 8.9％.The method was simple,sensitive,accurate and suitable for the quantify of 10 Sudan dyes in Paprika.关键词
超高效液相色谱/静电场轨道阱高分辨质谱/辣椒粉/苏丹类色素/快速筛查Key words
ultra high performance liquid chromatography/obitrap high-resolution mass spectrometry/paprika/sudan dyes/rapid screening分类
轻工纺织引用本文复制引用
王静,崔嘉,李兆杰,鞠玲燕,徐成钢,张慧敏,宋晓华,魏玮..超高效液相色谱-四极杆/静电场轨道阱高分辨质谱快筛查椒粉中10种苏丹类色素[J].中国食品添加剂,2018,(3):164-171,8.基金项目
国家质检总局科技计划项目(2016IK201). （2016IK201）
