食品工业科技2017，Vol.38Issue(1)：152-155,4.DOI:10.13386/j.issn1002-0306.2017.01.021
四株乳酸菌的加工性能
Processability of four strains of lactic acid bacteria
摘要
Abstract
Three kinds of common antibiotic,tetracycline,erythromycin and chloromycetin were selected for measuring the maximum tolerated concentration(MTC) of four strains of lactic acid bacteria in different antibiotics.The in vitro gastrointestinal environmental tolerance of the four strains of lactic acid bacteria were compared.Four strains of lactic acid bacteria had different levels of resistance to three kinds of antibiotics,and the maximum tolerated concentration were obtained:Leuconostoc mesenteroides subsp.Mesenteroides BD 1710,MTC of tetracycline and chloramphenicol was 1,2 μg/mL respectively;Lactobacillus plantarum ST-Ⅲ,MTC of chloramphenicol was 1 μg/mL;Lactobacillus casei BD0054,MTC of tetracycline and chloramphenicol was 1 μg/mL;Lactobacillus casei LC2W,MTC of chloramphenicol was 1 μg/mL.Four strains of lactic acid bacteria were intolerance to erythromycin.The results of simulated gastrointestinal environment experiment demonstrated:in the simulated gastric juice environment,the growth of ST-Ⅲ and LC2W were unaffected,great harm were caused to BD0054;Under the environment of simulated intestinal juice,the growth of ST-Ⅲ and BD1710 were unaffected,LC2W do not endure.When four strains of bacteria were treated with high temperatures,EDTA will enhance the damage to these bacteria.After freeze drying treatment added with 10％ skim milk,the survival rate were 90％ ～95％.关键词
乳酸菌/胃肠道耐受性/抗生素抗性/最高耐受浓度/EDTAKey words
Lactic acid bacteria/gastrointestinal tolerance/antibiotic resistance/maximum tolerated concentration/EDTA分类
轻工纺织引用本文复制引用
高彩霞,韩瑨,吴正钧..四株乳酸菌的加工性能[J].食品工业科技,2017,38(1):152-155,4.基金项目
国家“十二五”科技支撑计划(2013BAD18B01) （2013BAD18B01）
上海市科委项目(16DZ2280600) （16DZ2280600）
上海市闵行区领军人才项目(201443). （201443）
