食品工业科技2017,Vol.38Issue(1):156-159,164,5.DOI:10.13386/j.issn1002-0306.2017.01.022
肠道菌株单独及复合培养产雌马酚能力的评价
An assessment on the individual & collective equol cultivating productivity of intestinal bacteria
摘要
Abstract
Five common probiotics including Lacticacid bacteria,Lacticacid bacillus,Bifidobacterium,Bacillus subtilis,Streptococcus thermophilus and LJ-G1 and LJ-Q2 isolated from vegetarian feces were used as test bacteria.Isoflavone was added in the medium,the quantitative and qualitative analysis of the equol in medium was investigated by ultraviolet spectroscopy,which would be the basis of the ability to equlol transformability.Then the synergistic effects of the bacteria that can produce equol were inspected to a scale,and probe into the best culture condition of the compound bacteria.The results showed that Lacticacid bacteria,Streptococcus thermophilus,LJ-G1,LJ-Q2 can produced equol separately,and bacteria L J-G1,LJ-Q2 from vegetarian have a high capacity to produce equol with 15.92 μg/mL and 14.43 μg/mL.Lacticacid bacteria,they also had synergistic effects when they were recombined which produced equol between 19.04~20.78 μg/mL.The best composite ratio was 1:1,the pH of the medium was 7.0,culture temperature was 37 ℃,can produce equol was 21.23 μg/mg.The enteric bacteria from vegetarian feces can produce equol separately highly.关键词
肠道菌/雌马酚/大豆异黄酮/转化力Key words
intestinal bacteria/equol/soy isoflavone/transforming ability分类
轻工纺织引用本文复制引用
李笑梅,蒋梦涵,贾尧..肠道菌株单独及复合培养产雌马酚能力的评价[J].食品工业科技,2017,38(1):156-159,164,5.基金项目
黑龙江省自然科学基金项目(C201201) (C201201)
黑龙江省高校科技创新团队建设计划项目(2010td04). (2010td04)