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肠道菌株单独及复合培养产雌马酚能力的评价

李笑梅 蒋梦涵 贾尧

食品工业科技2017,Vol.38Issue(1):156-159,164,5.
食品工业科技2017,Vol.38Issue(1):156-159,164,5.DOI:10.13386/j.issn1002-0306.2017.01.022

肠道菌株单独及复合培养产雌马酚能力的评价

An assessment on the individual & collective equol cultivating productivity of intestinal bacteria

李笑梅 1蒋梦涵 1贾尧1

作者信息

  • 1. 哈尔滨商业大学黑龙江省高校食品科学与工程重点实验室,黑龙江哈尔滨150076
  • 折叠

摘要

Abstract

Five common probiotics including Lacticacid bacteria,Lacticacid bacillus,Bifidobacterium,Bacillus subtilis,Streptococcus thermophilus and LJ-G1 and LJ-Q2 isolated from vegetarian feces were used as test bacteria.Isoflavone was added in the medium,the quantitative and qualitative analysis of the equol in medium was investigated by ultraviolet spectroscopy,which would be the basis of the ability to equlol transformability.Then the synergistic effects of the bacteria that can produce equol were inspected to a scale,and probe into the best culture condition of the compound bacteria.The results showed that Lacticacid bacteria,Streptococcus thermophilus,LJ-G1,LJ-Q2 can produced equol separately,and bacteria L J-G1,LJ-Q2 from vegetarian have a high capacity to produce equol with 15.92 μg/mL and 14.43 μg/mL.Lacticacid bacteria,they also had synergistic effects when they were recombined which produced equol between 19.04~20.78 μg/mL.The best composite ratio was 1:1,the pH of the medium was 7.0,culture temperature was 37 ℃,can produce equol was 21.23 μg/mg.The enteric bacteria from vegetarian feces can produce equol separately highly.

关键词

肠道菌/雌马酚/大豆异黄酮/转化力

Key words

intestinal bacteria/equol/soy isoflavone/transforming ability

分类

轻工纺织

引用本文复制引用

李笑梅,蒋梦涵,贾尧..肠道菌株单独及复合培养产雌马酚能力的评价[J].食品工业科技,2017,38(1):156-159,164,5.

基金项目

黑龙江省自然科学基金项目(C201201) (C201201)

黑龙江省高校科技创新团队建设计划项目(2010td04). (2010td04)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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