食品工业科技2017,Vol.38Issue(1):160-164,5.DOI:10.13386/j.issn1002-0306.2017.01.023
基于磷脂脂肪酸分析技术的大曲微生物群落结构多样性研究
Studies on Daqu microbial community structure based on phospholipid fatty acid (PLFA)analysis
摘要
Abstract
The community diversity of microbial in sauce-flavor Daqu,strong-flavor Daqu and light-flavor Daqu were investigated using phospholipid fatty acids (PLFA) as a culture-independent method.The results showed that according to phospholipid fatty acid(PLFA) technique,a total of 18 fatty acids with chain lengths ranging from Ci4 to C20 were detected,including straight-chain PLFA,branched PLFA,monounsaturated PLFA,and the polyunsaturated PLFA.The amount of fungi dominated in the different Daqu.Specifically,gram-positive bacteria (G +) were the main bacteria,the 18∶2ω6,9 and 18∶1ω9 accounted for more than 90% PLFA.The correlation between the craftwork of Daqu and the microbial community structure was obvious.The community diversity was decreased as the craftwork temperature of Daqu increasing.关键词
大曲/磷脂脂肪酸/微生物群落Key words
Daqu/phospholipid fatty acid/microbial community分类
轻工纺织引用本文复制引用
赵金松,郑佳,沈才洪,周荣清..基于磷脂脂肪酸分析技术的大曲微生物群落结构多样性研究[J].食品工业科技,2017,38(1):160-164,5.基金项目
国家自然基金项目资助(31171742) (31171742)
四川理工学院人才引进项目(2014RC27). (2014RC27)