食品工业科技2017，Vol.38Issue(1)：208-213,6.DOI:10.13386/j.issn1002-0306.2017.01.032
琼胶寡糖的生物酶解制备工艺研究
Optimization of the preparation for the agar oligosaccharides by enzymatic hydrolysis
摘要
Abstract
The agar was obtained from Gracilaria lemaneiformis by water extraction.The optimal solvent-to-solid ratio,temperature and extraction time in the extration process were analysed by orthogonal test according to the yield of agar.Then the optimal pH value,hydrolysis temperature and substrate concentration in agar's enzymatic process were analysed by response surface method according to the degree of hydrolysis of agar.Result showed that the conditions containing solvent-to-solid ratio of 28∶1 (v/w),temperature of 120 ℃ and extraction time of 90 minutes was found to be optimal for extraction of agar and the yielf of agar was 32.8％.The condition cantaining pH value of 6.4,temperature of 54 ℃ and substrate concentration of 0.6％ (w/v)was found to be optimal for degrading agar and the degree of hydrolysis of agar was 89.75％.The products of enzymatic hydrolysis were determined as neoagarooligo saccharides with DPs of 2,4,6,and 8 by TLC,in which Neoagarotetraose was the major product,lay the foundation for the development and application of functional oligosaccharides agar.关键词
龙须菜/琼胶/琼胶寡糖/水提/酶解工艺Key words
Gracilaria lemaneiformis/agar/agar oligosaccharides/water extraction/enzymatical process分类
轻工纺织引用本文复制引用
谢喜珍,谢金盛,杨捷,叶秀云,林娟..琼胶寡糖的生物酶解制备工艺研究[J].食品工业科技,2017,38(1):208-213,6.基金项目
国家海洋公益性行业科研专项(201305015) （201305015）
福建省企业技术创新项目(海洋藻类资源高值化利用技术创新平台建设) （海洋藻类资源高值化利用技术创新平台建设）
福州市科技计划项目(2016X0005). （2016X0005）
