食品工业科技2017，Vol.38Issue(1)：287-290,295,5.DOI:10.13386/j.issn1002-0306.2017.01.049
采用HPLC同时进行连花清瘟颗粒多成分含量测定及其指纹图谱研究
Determination of multi components and analysis of fingerprint of Lianhua Qingwen granules by HPLC
摘要
Abstract
Objective:To establish a RP-HPLC method for the content determination of 4 active compounds in Lianhua Qingwen granules,including Phillyrin,Forsythoside A,Chlorogenic Acid,Crytochlorogenic Acid and fingerprint analysis.Methods:The separation was performed on Swell chromplus TM-C18 column with a temperature at 30 ℃,mobile phase consisted of methanol -acetonitrile-0.3％ phosphoric acid(gradient elution)at flow rate of 1.0 mL· min-1,and detection wavelength at 278 nm.Resuhs:The linear ranges were 0.01632～0.1632 mL· min-1 for phillyrin with the correlation coefficient of 0.9996,0.05512～0.5512 mL·min-1 for forsythoside A with the correlation coefficient of 0.9999,0.0188～0.188 mL·min-1 for chlorogenic acid with the correlation coefficient of 0.9998,0.011556～0.11556 mL·min-1 for crytochlorogenic acid with the correlation coefficient of 0.9998.The recovery were 110.57％ (RSD =2.76％,n =6),107.02％ (RSD =1.45％,n =6),108.59％ (RSD =1.78％,n =6),109.77％ (RSD =3.49％,n =6),respectively.And fingerprint spectrum was established.10 batches of samples were detected and 14 peaks in the chromatogram were common.There was a high similarity and each chromatographic peak was obtained with good separation.Conclusion:The method had a fine conclusion and can be used in quality control of Lianhua Qingwen granules.关键词
高效液相色谱法/连花清瘟颗粒/有效成分含量/指纹图谱Key words
HPLC/Lianhua Qingwen granules/content of active compound/fingerprint分类
轻工纺织引用本文复制引用
郭璐瑶,邓晶晶..采用HPLC同时进行连花清瘟颗粒多成分含量测定及其指纹图谱研究[J].食品工业科技,2017,38(1):287-290,295,5.基金项目
成都医学院2013年度“大学生创新实验计划”教师项目(CXJS201306). （CXJS201306）
