食品工业科技2018，Vol.39Issue(5)：106-109,118,5.DOI:10.13386/j.issn1002-0306.2018.05.020
罗布麻内生细菌的分离鉴定及抑菌活性
Isolation and identification of endophytic bacteria from Apocynum venetum L.and antibacterial activity
摘要
Abstract
Objective:To study the endophytic bacteria from Apocynum venetum Land their antibacterial activity in order to find novel resources.Methods:Endophytic bacteria were isolated from Apocynum venetum L.by using the method of plate cultivation,and the antibacterial activity of the fermented metabolites was studied by the Oxford Cup method.Meanwhile,the target endophytic bacteria were identified by 16S rRNA sequence homologous analysis combined with their physiological and biochemical characteristics.Results:The fermentation broths of endophytic bacteria showed different antibacterial activity.The strain D1 had inhibitory effect on Escherichia coli,Staphylococcus aureus,Micrococcus lutea and Bacillus subtilis,the strain D3 had inhibitory effect on Micrococcus lutea and Bacillus subtilis.The D1 was identified to be Pseudomonas hunanensis,and D3 was identified to be Pantoea ananatis.Conclusion:The results showed that Apocynum venetum L.contains abundant endophytic bacteria resources with antibacterial activity,which could be further developed and utilized.关键词
罗布麻/植物内生菌/抑菌活性Key words
Apocynum venetum L./endophyte/antibacterial activity分类
轻工纺织引用本文复制引用
彭秋萍,毛涵,代飞,杨智慧,汤新慧,薛菲..罗布麻内生细菌的分离鉴定及抑菌活性[J].食品工业科技,2018,39(5):106-109,118,5.基金项目
江苏省盐土生物资源研究重点实验室开放课题(JKLBS2013010) （JKLBS2013010）
盐城师范学院自然科学基金项目(16YCKLQ003) （16YCKLQ003）
国家级大学生创新训练计划项目(201610324003) （201610324003）
江苏省“青蓝工程”科技创新团队项目. （）
