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超声辅助酶法提取柚子皮多糖工艺的响应面优化

张荔菲 刘莹 王益 黄文

食品工业科技2018,Vol.39Issue(5):146-150,5.
食品工业科技2018,Vol.39Issue(5):146-150,5.DOI:10.13386/j.issn1002-0306.2018.05.028

超声辅助酶法提取柚子皮多糖工艺的响应面优化

Optimization of ultrasonic assisted enzyme method extraction technique on shaddock peel polysaccharides by response surface methodology

张荔菲 1刘莹 1王益 1黄文1

作者信息

  • 1. 华中农业大学食品科学技术学院,湖北武汉430070
  • 折叠

摘要

Abstract

Used shaddock peel as raw material to extract polysaccharides by ultrasonic assisted enzyme method via the response surface optimization.The results showed that the best extracting technological parameters of shaddock peel polysaccharides were as follows:pH6,solid to liquid ratio 1∶30 g/mL,enzyme amount 1.7%,enzyme ratio 1∶1,enzymolysis time 1.2 h,enzymolysis temperature 50 ℃,ultrasonic time 29 min,ultrasonic temperature 70 ℃.In this condition,the extraction rate of shaddock peel polysaccharides was 82.4%,the yield of polysaccharides was 27.3% and the purification rate was 46.2%.

关键词

柚子皮多糖/超声波辅助/纤维素酶/果胶酶/响应面法

Key words

shaddock peel polysaccharidess/ultrasonic-assisted extraction/cellulase/pectinase/response surface method

分类

轻工纺织

引用本文复制引用

张荔菲,刘莹,王益,黄文..超声辅助酶法提取柚子皮多糖工艺的响应面优化[J].食品工业科技,2018,39(5):146-150,5.

基金项目

农业部公益性行业(农业)科研专项项目(201503142). (农业)

食品工业科技

OA北大核心CSTPCD

1002-0306

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