食品工业科技2018，Vol.39Issue(5)：180-184,5.DOI:10.13386/j.issn1002-0306.2018.05.035
杨树口蘑多糖的超声波辅助提取工艺及其抗氧化活性
Optimization of ultrasonic-assisted extraction and antioxidant activity of polysaccharides from Tricholoma populinum
摘要
Abstract
The extraction and antioxidant activity of Tricholoma populinum polysaccharides (TPPs) were investigated in this study.Based on single factor experiments,the ultrasonic treating time,ultrasonic power and material to liquid ratio were analyzed and optimized by response surface methodology using TPPs yield as the response.Meanwhile,the antioxidant activities were evaluated by DPPH,hydroxyl and superoxide anion free radical scavenging capacity assays in vitro.The optimal extraction conditions were obtained as follows:extraction duration,27 min;ultrasonic power,410 W;and material to liquid ratio,1 ∶ 29 (g/mL).Under these conditions,the extraction yield of TPPs was 8.58％ ± 0.02％.The TPPs showed potential antioxidant activities in a concentration-dependent manner,the DPPH,hydroxyl and superoxidc anion free radical scavenging rates of TPPs were 52.25％,49.72％ and 58.24％ respectively at 0.05 mg/mL.The experimental results provide an experimental basis for studying the extraction and properties of TPPs.关键词
杨树口蘑/多糖/超声辅助提取/响应面/抗氧化Key words
Tricholoma populinum/polysaccharide/ultrasound-assisted extraction/response surface methodology/antioxidant activities分类
轻工纺织引用本文复制引用
郭华,刁全平,张博,侯冬岩..杨树口蘑多糖的超声波辅助提取工艺及其抗氧化活性[J].食品工业科技,2018,39(5):180-184,5.基金项目
辽宁省自然科学基金(2015020680) （2015020680）
鞍山市重大科技专项(20154818). （20154818）
