食品工业科技2018，Vol.39Issue(6)：126-129,4.DOI:10.13386/j.issn1002-0306.2018.06.023
一株高产木聚糖酶菌株的筛选鉴定及酶学特性研究
Screening,indetification and enzymatic properties of the high xylanase producing stain
摘要
Abstract
The strain RX-9 producing high xylanase was screened through preliminary screening and shake flask,based on enriched medium with xylan as the sole carbon source.The strain RX-9 had more than 99％ homology to Enterobacter,and was ascribed to Enterobacter sp.named as Enterbacter RX-9 by the colony morphology,physiological and biochemical analysis of experiment and genetic analysis of 16s rRNA sequence.The enzymatic properties of xylanase produced by the strain RX-9 was studied,and found that the enzyme activity was still 2310.03 U/mL kept at 100 ℃ for 30 min,and The xylanase activity was above 3200 U/mL when pH was 7.5～9.5.The results showed that the xylanase had good thermal stability and pH stability.关键词
木聚糖酶/筛选/鉴定/酶学特性Key words
xylanase/screening/identification/enzymatic properties分类
轻工纺织引用本文复制引用
郑虹,李莉娟,童秋霞,陈联邦,王艳君,季潇炜,邓加聪..一株高产木聚糖酶菌株的筛选鉴定及酶学特性研究[J].食品工业科技,2018,39(6):126-129,4.基金项目
福建省自然科学基金面上项目(2015J01132 （）
福建省教育厅中青年教师项目(JA15569) （JA15569）
,福建省国家自然基金应急管理项目(21547005). （21547005）
