食品工业科技2018，Vol.39Issue(6)：232-235,240,5.DOI:10.13386/j.issn1002-0306.2018.06.042
饮用水中亚硝酸盐高通量检测微孔板法的研究
Detection of nitritein in drinking water by high-throughput microplate method
摘要
Abstract
Under strong acid conditions,diazo-coupling reactions between nitrite and m-phenylenediamine generate dzoic compound,which is an orange-red compound.Based on this principle,a high-throughput microplate method was established using microplate as the reaction vessel.To improve the sensitivity of this method,several experimental parameters were optimized.The results revealed that the optimum measurement wavelength was 451 nm,and using 30 g/L m-phenylenediamine (0.4 mol/L hydrochloric acid as the solvent)as the chromogenic agent,the reaction was complete within 10 min at room temperature.The detection limit was 0.017 mg/L,and linear range was 0～5 mg/L,with correlation coefficient value of 0.9997.Recoveries of nitrite from watsons distilled water were tested from 95.24％ to 103.98％,with the coefficient of variation(CV) below 5％.This method was rapid,sensitive,easy to operate,and showed outstanding anti-interference ability,which was suitable for the screening of nitrite for a large number of drinking water samples.关键词
亚硝酸盐/间苯二胺/微孔板法/高通量/饮用水Key words
nitrite/m-phenylenediamine/microplate method/high-throughput/drinking water分类
轻工纺织引用本文复制引用
刘凤银,张媚健,李轩,谢丽君,袁学文,黄燕璇,梁岳,穆洪涛..饮用水中亚硝酸盐高通量检测微孔板法的研究[J].食品工业科技,2018,39(6):232-235,240,5.基金项目
国家自然科学基金(31601540) （31601540）
广东省自然科学基金(2016A030310304) （2016A030310304）
青年创新人才类项目(2015KQNCX106) （2015KQNCX106）
广东第二师范学院2014年博士科研专项经费研究项目(2014ARF02) （2014ARF02）
广东省食品质量安全重点实验室开放基金 （）
广东大学生科技创新培育专项资金项目(pdjh2017b0369). （pdjh2017b0369）
