食品科学2018，Vol.39Issue(5)：33-39,7.DOI:10.7506/spkx1002-6630-201805006
体外模拟消化液对金钗石斛的作用
Effect of Simulated Digestion in Vitro on Powders, Crude Extracts and Components of Dendrobium nobile Lindl.Stems
摘要
Abstract
The effects of three simulated digestive juices on the in vitro digestion of submicron and common powders,water and alcohol extracts,and crude dendrobine and polysaccharide from Dendrobium nobile Lindl.stems were investigated by using a model gastrointestinal system.The dissolution rates of dendrobine and polysaccharide were evaluated.By using high performance liquid chromatography (HPLC),3,5-dinitrosalicylic acid method and iron exchange chromatography,we determined the changes in the molecular weight,reducing sugar content and monosaccharide composition of polysaccharide after digestion.The polyphenols in digestive juices were analyzed by ultra-high performance liquid chromatographytandem mass spectrometry (UPLC-MS-MS).Results showed increasing dissolution rate of dendrobine during digestion.Submicron powder digestion showed the highest dendrobine dissolution rate of (21.46 ± 0.52)％.On the contrary,the lowest dissolution rate of (8.74 ± 0.50)％ was observed for the crude dendrobine.The dissolution rate of polysaccharide from the water extract was the highest among all the samples.Furthermore,during oral and gastric digestion,the submicron powder showed a higher dissolution rate of polysaccharide compared to the common powder,whereas the opposite was observed during intestinal digestion.In addition,simulated gastrointestinal digestion reduced the molecular weight of polysaccharide,and increased reducing sugar content,but did not produce any monosaccharides.The polyphenols from Dendrobium nobile Lindl.showed an increasing trend in simulated gastric juice,but generally decreased in simulated intestinal juice.关键词
金钗石斛/体外消化/石斛碱/多糖/多酚类化合物Key words
Dendrobium nobile Lindl./in vitro digestion/dendrobine/polysaccharides/polyphenols分类
轻工纺织引用本文复制引用
梅娜娜,娄在祥,王洪新,寇兴然,孟映霞..体外模拟消化液对金钗石斛的作用[J].食品科学,2018,39(5):33-39,7.基金项目
中央高校基本科研业务费专项资金项目(JUSRP51501) （JUSRP51501）
