食品科学2018，Vol.39Issue(5)：206-212,7.DOI:10.7506/spkx1002-6630-201805031
鲍内脏蛋白肽抗氧化和免疫调节活性
Antioxidant and Immunoregulatory Activity of Peptides from Abalone Visceral Protein Hydrolysate
摘要
Abstract
Peptides were prepared by enzymatic hydrolysis of abalone visceral proteins with alcalase followed by ultrafiltration.The molecular weights of most peptides were in the range of 192-938 Da as measured by high performance liquid chromatography (HPLC),accounting for 76.3％ of the total.The peptides were able to scavenge free radicals in vitro but the activity was much weaker than that of vitamin C (VC).The maximum percentage of DPPH radical scavenging activity was 32.7％,and the half maximal inhibitory concentration (IC50) for hydroxyl radical scavenging was 2.1 mg/mL.Furthermore,the peptides exhibited significant antioxidant activity in vivo by increasing the activity of superoxide dismutase (SOD) and the content of glutathione (GSH) in injured mice,and reducing the contents of malonaldehyde (MDA) and protein carbonyl group content in a dose-dependent manner.The peptides augmented SOD activity and GSH content by 33.2％ and 130％,and decreased MDA and protein carbonyl group content by 43.9％ and 40.7％,respectively.The effect was close to or even better than that of the positive control.The immunomodulatory activity assay proved that the peptides increased voix pedis swelling by 77.1％,antibody level by 28.0％,splenic index by 24.9％ and spleen index by 120％.Accordingly,we concluded that the peptides derived from abalone visceral proteins could enhance immune function in mice with respect to cellular immunity,humoral immunity,nonspecific immunity and immune organs and the effect was better than that of the positive control.关键词
鲍内脏/蛋白肽/抗氧化活性/免疫调节活性Key words
abalone viscera/peptides/antioxidant activity/immunoregulatory activity分类
轻工纺织引用本文复制引用
何传波,邵杰,魏好程,熊何健,吴国宏,马英,吴建勇..鲍内脏蛋白肽抗氧化和免疫调节活性[J].食品科学,2018,39(5):206-212,7.基金项目
国家海洋局海洋公益性行业科研专项(201405016) （201405016）
福建省科技引导性项目(2016N0022) （2016N0022）
福建省自然科学基金项目(2015J01141) （2015J01141）
