食品科学2018，Vol.39Issue(5)：213-219,7.DOI:10.7506/spkx1002-6630-201805032
小檗碱活化AMPK-eNOS减轻油酸所致人主动脉内皮细胞损伤
Protective Effect of Berberine on Oleic Acid-Induced Injury in Human Aortic Endothelial Cells via AMPK-eNOS Activation
摘要
Abstract
Objective:To investigate the protective effect and underlying mechanism of berberine chloride on oleic acid (OA)-induced damage in human aortic endothelial cells (HAECs).Methods:HAECs were treated with oleic acid alone or in combination with berberine,adenosine monophosphate-activated protein kinase (AMPK) activator AICAR (5-aminoimidazole-4-carboxamidel-β-D-ribofuranoside),or the AMPK inhibitor compound C,respectively.Cell proliferation was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay.Intracellular lipid accumulation was observed after Oil Red O staining and determined by triglycerides (TG) and total cholesterol (TC) assay kits.The secretion of nitric oxide (NO) by HAECs was detected by nitrate reductase assay.The phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS) was measured by Western Blotting assay.Results:OA exposure inhibited cell proliferation and resulted in lipid accumulation in HAECs in a concentration-dependent manner.Co-treatment with berberine resulted in no significant differences in all tested parameters compared with the normal control group and showed significantly improved cell viability compared with treatment with OA alone (P ＜ 0.01).The degree of lipid infiltration was higher after OA treatment compared with the normal control group.OA concentration-dependently induced lipid accumulation in endothelial cells,while lipid accumulation was significantly reduced by added berberine.OA significantly decreased NO levels compared with the normal control group,but this decrease was significantly reversed by berberine;furthermore,berberine repressed the increase caused by OA in reactive oxygen species (ROS).OA inhibited AMPK activation and consequently significantly attenuated the levels of p-AMPK and p-eNOS (P ＜ 0.01).Accordingly,berberine could reverse the reduced phosphorylation of AMPK and eNOS induced by OA,but this effect was antagonized by Compound C.Conclusion:Berberine can alleviate endothelial cell injury induced by oleic acid,which may be due to the activation of the AMPK-eNOS signaling pathway.关键词
小檗碱/油酸/单磷酸腺苷活化蛋白激酶/内皮型一氧化氮合酶/入主动脉内皮细胞Key words
berberine/oleic acid/adenosine monophosphate-activated protein kinase/nitric oxide synthase/human aortic endothelial cells分类
医药卫生引用本文复制引用
周慧,孙喜灵,王振华,刘敬,侯文锋,王尧清,张文彦,许波,马成俊,李忌,孟庆国..小檗碱活化AMPK-eNOS减轻油酸所致人主动脉内皮细胞损伤[J].食品科学,2018,39(5):213-219,7.基金项目
山东省泰山学者建设工程专项(tshw201502046) （tshw201502046）
国家自然科学基金面上项目(21372190 （）
81273628 （）
81473104) （）
中科院战略性先导科技专项(A类)子课题(XDA12040320) （A类）
烟台市科技计划项目(2014LGS004) （2014LGS004）
