食品科学2018，Vol.39Issue(5)：247-251,5.DOI:10.7506/spkx1002-6630-201805037
鸡卵类黏蛋白过敏原性的在体与离体实验
Allergenicity in Vivo and in Vitro of Ovomucoid
摘要
Abstract
This work was performed to study the allergenicity of ovomucoid (OVO) and to establish an animal model for this purpose.The OVO-induced anaphylactic shock in guinea pigs and the Schultz-Dale response of intestinal smooth muscle from sensitized guinea pigs when it was in vitro exposed to OVO were observed.The results obtained were as follows:(1) All the guinea pigs stimulated by ovalbumin at different dosages suffered from anaphylactic shock and finally died.The systemic allergic reactions were extremely positive (the score was 4 points).The systemic allergic reactions induced by ovomucoid were similar to those induced by 1 mg/mL ovalbumin (P ＞ 0.05) and were 100％ positive (the score was 2.04-3.68),causing the death of most of the animals.The effect of ovomucoid was dose dependent and an average score of 3.68 was observed at a dose of 4 mg/mL.(2) Ovomucoid was similarly effective as ovalbumin (P ＞ 0.05) in enhancing smooth muscle contraction in Schultz-Dale reaction.Muscle contractility showed the highest percentage of change (538.5％) when stimulated with 2 mg/mL of ovomucoid.(3) Serum immunoglobulin G (IgG) levels of guinea pigs stimulated by ovalbumin and ovomucoid were both obviously higher than those of the control group and their effects were dose dependent.In conclusion,the animal model developed (in vivo and in vitro) in this research allowed to detect the allergenicity of OVO and evaluate its elimination and reduction.关键词
卵类黏蛋白/卵白蛋白/动物模型/过敏性休克/Schultz-Dale反应Key words
ovomucoid/ovalbumin/animal model/anaphylactic shock/Schultz-Dale reaction分类
医药卫生引用本文复制引用
王丽娟,吴子健,刘露,陈一华,裴高鑫,王勇..鸡卵类黏蛋白过敏原性的在体与离体实验[J].食品科学,2018,39(5):247-251,5.基金项目
天津市应用基础与前沿技术研究计划面上项目(14JCYBJC30900) （14JCYBJC30900）
