食品科学2018，Vol.39Issue(5)：252-257,6.DOI:10.7506/spkx1002-6630-201805038
东北山核桃壳黄酮对小鼠氧化损伤的防御作用
Protective Effect of Flavonoids from Juglans mandshurica Seed Shells Grown in Northeast China against Oxidative Damage in Mice
摘要
Abstract
The protective effect of flavonoids from seed shells of Juglans mandshurica (FSSJM) against oxidative damage in mice was explored.A mouse model of oxidative damage was built by X-ray radiation.Before radiation,using berbamine hydrochloride gavaged at 56 mg/(kg.d) as the positive control,FSSJM were consecutively given to mice by gavage at three different doses of 100,200 and 400 mg/(kg·d) for 14 days.The activities of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and catalase (CAT),and the contents of malondialdehyde (MDA) and glutathione (GSH) in serum and liver tissues of 24 h radiated mice were determined by spectrophotometry.Liver tissues of 14 day radiated mice were observed morphologically,and the expression of Bax and Bcl-2 protein in liver and spleen were analyzed by immunohistochemisty.The results showed that FSSJM could increase the activities of SOD,GSH-Px and CAT in serum and liver of mice,decrease MDA content,and increase GSH content.Furthermore,FSSJM could repair damaged liver tissues,along with increasing Bcl-2 protein expression and decreasing Bax protein expression.Therefore,FSSJM can protect against radiation-induced oxidative damage.关键词
东北山核桃壳/黄酮/辐射/氧化损伤/防御Key words
Juglans mandshurica seed shells grown in Northeast China/flavonoids/radiation/oxidative damage/protection分类
农业科技引用本文复制引用
张金堂,沙迪,夏广军,徐红艳..东北山核桃壳黄酮对小鼠氧化损伤的防御作用[J].食品科学,2018,39(5):252-257,6.基金项目
吉林省科技发展计划项目(20150204063NY) （20150204063NY）
