食品科学2018，Vol.39Issue(6)：75-81,7.DOI:10.7506/spkx1002-6630-201806013
环境胁迫对屎肠球菌8-3LuxS/AI-2群体感应系统的影响
Effects of Environmental Stress on LuxS/AI-2 Quorum Sensing Systerm of Enterococcus faecium 8-3
摘要
Abstract
Enterococcusfaecium 8-3,which was isolated from koumiss and screened for autoinducer-2 (AI-2),was used to study the effects of growth conditions (pH value,temperature,osmotic pressure,and nutrient condition) on the LuxS/AI-2 quorum sensing system.The production of AI-2 was detected by a biological method and the expression levels of luxS and pfs genes were detected by real-time quantitative PCR.The results showed that mild acid stress induced E.faecium 8-3 to produce the AI-2.Under alkaline conditions,the cell grew better,and the cell density mainly induced the production of AI-2.Under osmotic and low temperature stresses,the growth of E.faecium 8-3 and the production of AI-2 were both inhibited.Cell density controlled AI-2 production.Low nutrient stress could induce E.faecium 8-3 to produce AI-2.The expression of luxS and pfs genes in E.faecium 8-3 under different environmental stresses varied to different degrees,indicating that the LuxS/AI-2 quorum sensing system mediates stress resistance in E.faecium 8-3.关键词
群体感应/自体诱导物-2/环境胁迫/基因表达Key words
quorum sensing/autoinducer-2/environmental stress/gene expression分类
轻工纺织引用本文复制引用
顾悦,李博,吴荣,田建军,吴敬,贺银凤..环境胁迫对屎肠球菌8-3LuxS/AI-2群体感应系统的影响[J].食品科学,2018,39(6):75-81,7.基金项目
国家自然科学基金地区科学基金项目(31360396) （31360396）
