食品科学2018，Vol.39Issue(6)：88-94,7.DOI:10.7506/spkx1002-6630-201806015
降胆固醇乳酸菌的体外筛选及其降胆固醇机理探讨
In Vitro Screening of Lactic Acid Bacteria for Cholesterol-Lowering Activity and the Underlying Mechanism
摘要
Abstract
Objective:To screen 29 Lactobacillus strains isolated from kefir grains and fermented pickles for cholesterol-lowering activity and to explore the mechanism of action of the selected isolates in degrading serum cholesterol in rats.Methods:One of these isolates,identified as Lactobacillus plantarum DMDL 9010,was selected for its acid resistance,bile salt tolerance,hydrophobicity,bile salt hydrolase activity and cholesterol-lowering property.A feeding experiment was conducted on fifty eight-week-old Sprague Dawley rats,which were randomly divided into normal diet control,hyperlipidaemic diet control,positive (atorvastatin calcium) control,high-dose and low-dose DMDL 9010 groups.The rats were fed for 10 weeks.The serum levels of total cholesterol (TC),triglyceride (TG),high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were determined on the 28th and 70th day of the experiment.The real-time fluorescence reverse transcription PCR was used to detect the expression level of 3-hydroxy-3-methylglutaryl-CoA reducase (HMGCR) as the rate-limiting enzyme for cholesterol synthesis.Results:L.plantarum DMDL 9010 was capable of 37.58％ degradation of cholesterol and showed 35.48％ tolerance to bile salt and a hydrophobicity of up to 40％.It had good acid resistance.A hypercholesterolemic rat model was successfully constructed after feeding a high-cholesterol diet for 28 days.Administration of high-dose DMDL 9010 for 70 days significantly reduced serum TC (23.03％) and LDL-C (28.00％),but there was no significant difference between the low-dose and positive control groups.The positive control and high-dose groups significantly down-regulated the expression level of HMG-CoA mRNA in the liver (79.92％ and 62.86％,respectively) (P ＜ 0.05).Conclusion:It is possible to develop L.plantarum DMDL 9010 into a functional microecological agent.关键词
降胆固醇/乳酸菌/分离/鉴定/3-羟基-3-甲基戊二酰辅酶A还原酶Key words
cholesterol-lowering/Lactobacillus/isolate/identification/HMGCR分类
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黄燕燕,郭均,黎恒希,杨爱君,冯立科,彭小霞,刘冬梅..降胆固醇乳酸菌的体外筛选及其降胆固醇机理探讨[J].食品科学,2018,39(6):88-94,7.基金项目
国家自然科学基金面上项目(31771908) （31771908）
广东省自然科学基金项目(S2011010005679) （S2011010005679）
广东省省级科技计划项目(2013B020312002 （）
2014A020208019) （）
广东燕塘乳业股份有限公司委托项目(D8133540) （D8133540）
