食品科学2018，Vol.39Issue(6)：95-100,6.DOI:10.7506/spkx1002-6630-201806016
乳酸对阴沟肠杆菌的抑菌作用及其机制分析
Antimicrobial Activity and Mechanism of Lactic Acid on Enterobacter cloacae
摘要
Abstract
The antimicrobial activity of lactic acid (LA) against a dominant strain of Enterobacter cloacae isolated from spoiled frozen chicken was examined in the study.The results showed that all the selected LA solutions (1.0％,0.5％,and 0.25％) had bactericidal action against E.cloacae.The cell membrane potential and the leakage of intracellular ATP and ultraviolet-absorbing substances were increased in cells treated with 1.0％ LA.The treated cells showed an irregular morphology under transmission electron microscope (TEM);furthermore,the cell wall was damaged and the intracellular contents were leaked out.The permeability of the cytoplasmic membrane was increased as shown by laser scanning confocal microscope and flow cytometry.Therefore,the bactericidal effect of LA against E.cloacae was mainly mediated by damaging the cell wall and consequently increasing the cell membrane permeability,thereby leading to leakage of the intracellular components.关键词
乳酸/阴沟肠杆菌/抑菌机制Key words
lactic acid/Enterobacter cloacae/antibacterial mechanism分类
轻工纺织引用本文复制引用
王凤婷,孙芝兰,吴海虹,许晓曦,刘芳,王道营,耿志明,诸永志,徐为民..乳酸对阴沟肠杆菌的抑菌作用及其机制分析[J].食品科学,2018,39(6):95-100,6.基金项目
国家自然科学基金面上项目(31371802) （31371802）
江苏省自然科学基金面上项目(BK20151367) （BK20151367）
江苏省产学研项目(BY2015073) （BY2015073）
