食品科学2018，Vol.39Issue(6)：122-129,8.DOI:10.7506/spkx1002-6630-201806020
基于面包酵母中海藻糖提取工艺优化及菌株筛选的高密度培养工艺
Optimization of Extraction Process for Trehalose from Baker's Yeast for Strain Screening and High Density Culture
摘要
Abstract
The microwave-assisted extraction of trehalose was optimized using one-factor-at-a-time and orthogonal array design methods.Intracellular trehalose in yeast cells was determined by 3,5-dinitrosalicylic acid (DNS) method after hydrolysis by trehalase.The optimum conditions for extracting trehaiose from 1.5 g of active dry yeast were determined to be extraction at 55 ℃ for 150 min using 40 mL of 0.7 mol/L trichloracetic acid as the extraction solvent with microwave treatment duration of 40 s at 231 W power.Under these conditions,the yield of trehalose was 280.15 mg/g,15.2％ higher than that before optimization.Furthermore,one of the 10 baker's yeast strains preserved in our laboratory was selected for high yield production of trehalose.In a 50 L self-priming fermenter,the selected strain was used to perform fed-batch fermentation.The maximum wet yeast biomass was 198.34 g/L by using feedback control of dissolved oxygen concentration through feeding molasses.By feeding a predetermined amount of molasses,urea and phosphoric acid as nutrient source,the maximum wet yeast biomass was increased to 264.82 g/L,which was increased by up to 33.52％ compared with the original procedure and suggested high density culture of baker's yeast.In the fermentation process,the alcohol content (V/V) was controlled between 0.7％ and 1.0％,thereby effectively improving wet yeast biomass and simultaneously ensuring that the fermentation power is over 650 mL/h,and the modified process was confirmed to stable.关键词
面包酵母/海藻糖/称质量补料/乙醇体积分数Key words
baker's yeast/trehalose/weighing feeding/alcohol content分类
轻工纺织引用本文复制引用
洪厚胜,窦冰然,郭会明..基于面包酵母中海藻糖提取工艺优化及菌株筛选的高密度培养工艺[J].食品科学,2018,39(6):122-129,8.基金项目
国家高技术研究发展计划(863计划)项目(2012AA021201) （863计划）
