食品科学2018，Vol.39Issue(6)：270-275,6.DOI:10.7506/spkx1002-6630-201806042
响应面法优化蛹虫草固态发酵产物虫草素与腺苷综合提取工艺
Optimization of Simultaneous Extraction of Cordycepin and Adenosine Produced by Solid-State Fermentation with Cordyceps militaris by Response Surface Methodology
摘要
Abstract
Cordycepin and adenosine were simultaneously extracted from the solid-state culture of Cordyceps militaris with rice and brewer's spent grain (BSG) as co-substrates.The extraction process was optimized using one-factor-at-a time method and response surface methodology (RSM).The total yield of cordycepin and adenosine was investigated as a function of four variables including extraction time,ethanol concentration,solvent-to-solid ratio and temperature.The optimal extraction conditions were found to be extraction at 45 ℃ for 87 min with water as a solvent at a solvent-to-solid ratio of 22∶1 (mL/g).Under these conditions,The maximum total yield (2.491‰o) of cordycepin and adenosine was experimentally obtained,which agreed with the predicted value (2.487‰).Accordingly,the regression model developed showed excellent goodness of fit.The solid-state culture of Cordyceps militaris is a promising rich of cordycepin and adenosine.关键词
蛹虫草/固态发酵产物/综合提取/响应面法Key words
Cordyceps militaris/solid-state culture/simultaneous extraction/response surface methodology分类
农业科技引用本文复制引用
于戈,王丽,胡欣蕾,孙亚男,张欣,李铭,李文香..响应面法优化蛹虫草固态发酵产物虫草素与腺苷综合提取工艺[J].食品科学,2018,39(6):270-275,6.基金项目
山东省现代农业产业技术体系建设经费资助项目(SDAIT-07-07) （SDAIT-07-07）
山东省科技发展计划项目(2014GNC113007) （2014GNC113007）
