食品科学2018，Vol.39Issue(6)：284-290,7.DOI:10.7506/spkx1002-6630-201806044
辽宁地区绿豆品种SSR指纹图谱构建及品种鉴定
Establishment of Simple Sequence Repeat Fingerprint for Identification of Mungbean Varieties (Vigna radiate L.) Grown in Liaoning
摘要
Abstract
Objective:This study aimed to establish a DNA fingerprint for rapid and accurate identification of mung bean varieties grown in Liaoning.Methods:A total of 48 mung bean samples from different varieties were used to construct a fingerprint database and a DNA fingerprint with 15 pairs of polymorphic simple sequence repeat (SSR) primers.Results:A total of 58 alleles were detected,2-7 of which,with an average of 3.9,were detected with each pair of the primers used,and 13 specific bands were obtained,accounting for about 22.4％ of the total.The polymorphic information content (PIC) varied from 0.114 5-0.776 4,with an average of 0.378 4.Five pairs of efficient primers were selected.The discrimination rate obtained with their combination was as high as 75％,while a discrimination rate of 89.68％ was obtained with a combination of all 15 primer pairs.Based on these results,the DNA fingerprint database composed of the digits 0 and 1 and the SSR fingerprint were established,which allowed unequivocal identification of all the varieties except a few unique ones.Conclusion:The SSR fingerprint presented in this study can open up a new way of identifying mung bean varieties and provide a basis for mung resource evaluation and protection as well as breeding of new varieties.关键词
SSR指纹图谱/绿豆/品种鉴定Key words
SSR fingerprinting/mung bean/variety identification分类
农业科技引用本文复制引用
赵雅楠,王颖,张东杰..辽宁地区绿豆品种SSR指纹图谱构建及品种鉴定[J].食品科学,2018,39(6):284-290,7.基金项目
“十二五”农村领域国家科技支撑计划项目(2012BAD34B02) （2012BAD34B02）
黑龙江省应用技术与开发重大项目(GA14B104) （GA14B104）
大庆科技局创新项目(sjh-2013-65) （sjh-2013-65）
黑龙江省研究生创新科研项目(YJSCX2017-Y50) （YJSCX2017-Y50）
