食品科学2018，Vol.39Issue(6)：298-301,4.DOI:10.7506/spkx1002-6630-201806046
还原糖快速检测试纸的研制
Development of a Rapid Strip Test for Detection of Reducing Sugar
摘要
Abstract
A rapid strip test for the detection of reducing sugar was developed based on the fact the chromogenic reaction of reducing sugar and 3,5-dinitrosalicylic acid (DNS) is triggered by the alkaline environment and the heat generated by the reaction between calcium oxide and aqueous samples.The experimental conditions were determined and the detection range of the test system was investigated.Standard colorimetric card was prepared.The results showed that the best base paper was rapid qualitative filter paper,the concentration of chromogenic agent was 6.0 g/L and drying temperature was 50 ℃.The test required only 5 min and the detection range was 0.01-3.00 mol/L.This method had the advantages of simple operation,time saving,rapid color development and convenience.It was suitable for fast semi-quantitative detection of reducing sugar in food and medicinal fields and fermentation industry.关键词
3,5-二硝基水杨酸比色法/试纸/还原糖Key words
DNS colorimetry/strip test/reducing sugar分类
轻工纺织引用本文复制引用
刘萧,马千里,杨仁党..还原糖快速检测试纸的研制[J].食品科学,2018,39(6):298-301,4.基金项目
浙江省农产品化学与生物加工技术重点实验室浙江省农业生物资源生化制造协同创新中心开放基金项目(2016KF0201) （2016KF0201）
中央高校基本科研业务费专项(x2qs-D2171020) （x2qs-D2171020）
制浆造纸工程学科引智基地项目(2015B020241001) （2015B020241001）
