食品与发酵工业2018，Vol.44Issue(2)：62-67,6.DOI:10.13995/j.cnki.11-1802/ts.015176
小曲中优质产酯酵母分离鉴定及其产酯条件优化
Isolation and identification of efficient ester-producing yeast in Xiaoqu and optimization of ester production conditions
摘要
Abstract
An efficient ester-producing yeast (numbered as Y5) was isolated from three kinds of Xiaoqu and identified as Wickerhamomyces anomalus by physiological biochemical tests and molecular biology method.Using esterproducing culture-medium,2.481 g/L of ethyl acetate among 2.684 g/L of total ester was obtained.Using orthogonal experiments to optimize ester-production conditions,results showed that the appropriate culture conditions for this yeast was fermentation in alcohol 4％,pH 4,at 30 ℃ for 3 d.Under this condition,total ester and ethyl acetate was 4.065 g/L and 3.677 g/L respectively,which was significantly increased about 51.5％ and 48.2％ respectively compared that before optimization.The strain has strong ability to produce ester and there is a bright prospect for its application in industry.关键词
小曲/异常威克汉姆酵母/总酯/乙酸乙酯/产酯条件Key words
Xiaoqu/Wickerhamomyces anomalus/ester content/ethyl acetate/production conditions引用本文复制引用
王鹏昊,许琴,关统伟,张习超,赵顺先,向慧平,张家旭,赵小林,欧梦莹,林宜锦..小曲中优质产酯酵母分离鉴定及其产酯条件优化[J].食品与发酵工业,2018,44(2):62-67,6.基金项目
国家自然科学基金(31060003) （31060003）
企业委托项目(16205257、16205231、172243、15205213)资助 （16205257、16205231、172243、15205213）
