食品与发酵工业2018，Vol.44Issue(2)：92-97,6.DOI:10.13995/j.cnki.11-1802/ts.014818
草鱼鳞和草鱼皮胶原蛋白性质及自聚集行为对比研究
Comparative study on characteristics and self-assembly behavior of collagens extracted from skins and scales of grass carp
摘要
Abstract
Collagens were extracted from the grass carp skin and scale by acid-enzyme combination method.Their structural properties were analyzed by ultraviolet spectrum (UV),sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE),fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC).Self-assembly behavior of collagens were compared by turbidity experiment,fibril formation detection and scanning electronic microscopy (SEM).The results indicated that collagens from grass carp skin and scale were characterized as type Ⅰ collagen with two α-chains and a triple helix structure.Denaturation temperatures of the collagens were 34.99 ℃ and 39.75 ℃,respectively.The self-assembly curves showed that the collagens could self-assemble into fibrils at 30 ℃ near neutral pH with NaC1,and self-assembly degree of collagens were similar from skin 28％ and scale 27.33％.Scanning electronic microscopy observations suggested that both collagens could form intertwine fibril network.The reticular fibrous structure of scale collagen was more obvious than skin collagen,and a periodic striped D-band was observed,while the skin collagen fiber structure had a collapse without D-band.关键词
草鱼皮/草鱼鳞/胶原蛋白性质/自聚集Key words
grass carp skin/grass carp scale/collagen characteristics/self-assembly引用本文复制引用
兰文婷,金若芸,刘耀文,王淑瑶,马东辉,何利..草鱼鳞和草鱼皮胶原蛋白性质及自聚集行为对比研究[J].食品与发酵工业,2018,44(2):92-97,6.基金项目
四川省科技厅重点研发项目(2017GZ0428) （2017GZ0428）
四川省教育厅项目(16ZB0044,035Z1373) （16ZB0044,035Z1373）
