食品与发酵工业2018，Vol.44Issue(2)：172-175,181,5.DOI:10.13995/j.cnki.11-1802/ts.015839
黄酮“落新妇苷”对胰脂肪酶抑制作用研究
The inhibiting effect of flavonoid "astilbin" on pancreatic lipase
摘要
Abstract
In this paper,we studied the inhibitory activity of astilbin on pancreatic lipase.The results showed that astilbin had inhibitory effect on pancreatic lipase with IC50 of 105 μmol/L and maximum inhibition rate of 56.3％.The inhibitory effect increased with the interaction time between astilbin and pancreatic lipase.Enzyme kinetics study showed that astilbin was a noncompetitive inhibitor of pancreatic lipase,which did not change its affinity with the substrate,but decreased its maximum reaction rate.The fluorescence spectra showed that astilbin could quench the endogenous fluorescence of pancreatic lipase,and made its maximum emission wavelength red shift.Their binding constant (lgKa) was 5.50 and the binding-site number was 1.17.关键词
落新妇苷/胰脂肪酶/抑制作用Key words
astilbin/pancreatic lipase/inhibiting effect引用本文复制引用
郑丹,张清峰..黄酮“落新妇苷”对胰脂肪酶抑制作用研究[J].食品与发酵工业,2018,44(2):172-175,181,5.基金项目
国家自然科学基金项目(31760461) （31760461）
江西省教育厅基金项目(GJJ160416) （GJJ160416）
江西省天然产物与功能食品重点实验室开放基金项目(2015003) （2015003）
