食品与机械2018，Vol.34Issue(2)：1-5,5.DOI:10.13652/j.issn.1003-5788.2018.02.001
外源转基因片段从转基因棉籽壳到食用菌水平转移研究
Study on the transferring of the gene fragment from the genetical ly modified cottonseed hulls to the edible fungi
摘要
Abstract
It is a question in the field of food-safety that whether for-eign genetically modified genes would transfer from the genetically modified cottonseed hulls to edible fungi.In this study,1 5 Tremella fuciformis samples and 10 Pleurotus ostreatus samples (the cultiva-tion media contained 75% and 92% genetically modified cottonseed hulls,respectively)collected from cultivation meida were evaluated by PCR to confirm whether the foreign genetically modified genes, CaMV 35S,NOS,CpTⅠ,Cry IA(c),were present.In addition, 47 samples which were probably grown in the media containing cot-tonseed hulls,were collected from the large and medium-sized super-markets and farm produce markets.All of the results of PCR showed that the foreign sequences were not amplified by PCR.In conclusion, No exogenous transgenic fragments in the genetically modified cot-tonseed hulls was found transferred to the edible fungi.关键词
转基因棉籽壳/银耳/平菇/食用菌/基因水平转移/PCRKey words
genetically modified cottonseeds hulls/edible fungi/Tremella fuciformis/Pleurotus ostreatus/gene transferring/PCR引用本文复制引用
白亚龙,索玉娟,林婷,沈源源,赵晓燕,刘海燕,周昌艳..外源转基因片段从转基因棉籽壳到食用菌水平转移研究[J].食品与机械,2018,34(2):1-5,5.基金项目
国家农产品质量安全风险评估项目(编号:GJFP2017006) （编号:GJFP2017006）
上海市农委(兴农推广-成长计划)[编号:沪农青字(2017)第1-28号] （兴农推广-成长计划）
