食品与机械2018，Vol.34Issue(2)：6-10,82,6.DOI:10.13652/j.issn.1003-5788.2018.02.002
马铃薯蛋白ACE抑制肽的Plastein反应修饰研究
Plastein reaction to modify the ACE inhibitory peptides of potato protein
摘要
Abstract
Potato protein was hydrolyzed by trypsin to prepare for ACE inhibitory peptides in our previous researches (the ACE inhibi-tion rates was 61.20%±0.15%),and we used plastein reaction to modify potato protein hydrolysates for enhancing the ACE inhibitory activity of peptides in this study.The influences of the kinds of added amino acids,the substrate/enzyme ratio,pH value,the temperature and hydrolysis time on ACE inhibitory activity of the plastein reaction modification of potato protein hydrolysates were investigated.To achieve the maximum ACE inhibitory activity in the plastein reaction modification of cottonseed protein hydrolysates,the response surface methodology was applied to find the optimum condi-tions based on the single factor tests.[E]/[S]ratio at 3 565.05 U/g, substrate concentration at 30.0%,and extrinsic dose of glycine at 0.5 mol/g reacted at 43.68 ℃ and pH 8.05 for 3.0 h,were found to be the optimal conditions,and the ACE inhibitory activity could reached (82.89±0.05)%.Compared with unmodified potato ACE inhibitory peptide,its ACE inhibitory activity increased by 1.3 5 times.关键词
马铃薯蛋白/ACE/肽/类蛋白反应Key words
The potato protein/ACE/peptide/plastein reaction引用本文复制引用
高丹丹,马忠仁,热孜万古力·赛买提,田晓静,李明生,陈士恩..马铃薯蛋白ACE抑制肽的Plastein反应修饰研究[J].食品与机械,2018,34(2):6-10,82,6.基金项目
国家自然科学基金计划(编号:31360375) （编号:31360375）
西北民族大学"双一流引导专项"生物工程特色学科建设(编号:10018704) （编号:10018704）
西北民族大学中央高校基本科研业务费专项资金(编号:31920170178) （编号:31920170178）
甘肃省科技计划资助(编号:1504WKCA094) （编号:1504WKCA094）
科技部援助项目(编号:KY201501005) （编号:KY201501005）
甘肃省兰州市城关区科技计划项目(编号:2017KJGG0024) （编号:2017KJGG0024）
甘肃省科技计划资助(编号:17YF1WA166) （编号:17YF1WA166）
"长江学者和创新团队发展计划"创新团队(编号:IRT_17R88) （编号:IRT_17R88）
