食品与机械2018，Vol.34Issue(2)：15-18,4.DOI:10.13652/j.issn.1003-5788.2018.02.004
桑白皮多酚的抗氧化和对UV辐射致成纤维细胞光老化的修复作用
Antioxidant and UV-induced fibroblasts photoaging repair effects of polyphenol from Cortex Mori
摘要
Abstract
The antioxidant activity of polyphenol from Cortex Mori(CMP)was examined by 2,2'-azinobis-(3-ethylbenzthiazoline-6-sul-phonate (ABTS)and 1,1-diphenyl-2-picrylhydrazyl (DPPH)free radical scavenging assays.Fibroblasts HS68 cells were induced by ul-traviolet (UV)irradiation to establish the photoaging model.Cell proliferation was investigated using a methyl thiazolyl tetrazolium (MTT)assay.The content of procollagen typeⅠprotein (PIP)in cells was measured by enzyme-linked immunosorbent assay (ELISA). The matrix metalloproteinase-1 (MMP-1)and PIP mRNA expressi-ons were detected by qRT-PCR.CMP exhibited significant antioxi-dant activity in ABTS,DPPH free radical scavenging assays,with IC 50values of 1.49,8.94 μg/mL,respectively.The IC 50values of butyl hydroxy anisd (BHA)against ABTS,DPPH radicals were 11.25,11.03 μg/mL,respectively.The results showed that CMP had stronger scavenging effect on free radicals,and its clearance rate was higher than that of BHA.Compared with the UV group,CMP not only significantly enhanced the fibroblasts proliferation (P<0.05),but also dose-dependently increased the content of PIP (P<0.05).At the same time,CMP down-regulated the MMP-1 mRNA level,and promoted the mRNA expression of PIP (P<0.05).To conclude,polyphenol from Cortex Mori could repair fibroblasts a-gainst UV-induced photoaging,and its mechanism may be related to its scavenging effect on free radicals,the inhibition of the expression of MMP-1,and the regulation of the synthesis of PIP.关键词
桑白皮/多酚/抗氧化/成纤维细胞/光老化Key words
Cortex Mori/polyphenol/antioxidant/fibroblasts/pho-toaging引用本文复制引用
吴永祥,吴丽萍,王卫东,金泰完..桑白皮多酚的抗氧化和对UV辐射致成纤维细胞光老化的修复作用[J].食品与机械,2018,34(2):15-18,4.基金项目
安徽省高校自然科学研究重点项目(编号:KJ2017A398) （编号:KJ2017A398）
安徽省留学回国人员创新项目择优资助计划重点项目(编号:2017srst1) （编号:2017srst1）
校企合作项目(编号:hxkt20170015,hxkt20170016) （编号:hxkt20170015,hxkt20170016）
