食品与机械2018，Vol.34Issue(2)：19-24,6.DOI:10.13652/j.issn.1003-5788.2018.02.005
EMS-ARTP复合诱变选育高产DHA裂殖壶菌
Enhancement of docosahexaenoic acid production in Schizochytrium sp. 31 combining Ethylmethylsulfone and Atmospheric and Room Temperature Plasma treatment
摘要
Abstract
For establishing an efficient and stable mutagenesis method on breeding Schizochytrium DHA higher producers,the wild-type strain named Schizochytrium sp.31 was mutated combining Ethylm-ethylsulfone (EMS) and Atmospheric and Room Temperature Plasma (ARTP)treatment.Mutagenic efficiency,fermentation inde-xes of the mutants were compared and analyzed.Under this compound mutagenesis method,the positive mutation rate reached 32.2%,which was much higher than that under other two single-fac-tor mutagenesis methods.Besides,DHA production and DHA per-centage in total fatty acids reached to 7.2 g/L and 43.2%,which were 35.6% and 19.2% higher than that of the wild-type strain,re-spectively.After five rounds of cell culture passages,mutants induced from compound mutagenesis method showed up with better genetic stability.This mutagenesis method was proved to be an effi-cient strategy of breeding Schizochytrium DHA higher producers.It also provideed references to the breeding practices on the algal higher producers for synthesizing other types of polyunsaturated fatty acids.关键词
裂殖壶菌/DHA/诱变/常压室温等离子体(ARTP)/甲基磺酸乙酯(EMS)/复合诱变Key words
Schizochytrium/docosahexaenoic acid/mutagenesis/at-mospheric and room temperature plasma/ethyl methanesulfonate/compound mutagenesis引用本文复制引用
赵犇,王武,李昌灵,杨海麟..EMS-ARTP复合诱变选育高产DHA裂殖壶菌[J].食品与机械,2018,34(2):19-24,6.基金项目
国家"863"计划(编号:2014AA021702) （编号:2014AA021702）
江苏省2014年度普通高校研究生科研创新计划项目(编号:KYLX_1148) （编号:KYLX_1148）
工业生物技术教育部重点实验室2016年度开放课题(编号:KLIB-KF201601) （编号:KLIB-KF201601）
"111"计划(编号:111-2-06) （编号:111-2-06）
