食品与机械2018，Vol.34Issue(2)：149-153,175,6.DOI:10.13652/j.issn.1003-5788.2018.02.032
龙须菜挥发油的提取及抗菌抗虫活性研究
Extract of volatile oil and antibacterial & insecticidal activity of large red algae GraciIaria Iemaneiformis
摘要
Abstract
The volatile oil of Gracilaria lemaneiformis was extracted by direct steam distillation and solvent extraction-steam distillation, meanwhile gas chromatography-mass spectrometry (GC-MS)and antibacterial & insecticidal activity were conducted.The GC-MS showed that compared with direct steam distillation extraction,in solvent extraction-steam distillation extraction the relative content of aldehyde compounds which has lower odor threshold is 0.20%, meanwhile the relative content of carboxylic acid and ester is 21.54%in solvent extraction-steam and distillation extraction.The antibacte-rial activity of volatile oil on 5 kinds of pathogens (Escherichia coli O157,Staphylococcus aureus,Salmonella enterica,Listeria mono-cytogenes,Vibrio parahemolyticus)is moderate,and the median le-thal dose LD 50of volatile oil compounds in G.Lemaneiformis with solvent extraction-steam distillation and direct steam distillation broth were 0.126 and 0.162 mg/mL respectively.关键词
龙须菜/挥发油/抗菌/抗虫/水蒸气蒸馏法/溶剂提取-水蒸气蒸馏法/气相色谱-质谱法Key words
Gracilaria lemaneiformis/volatile oil/antibacterial ac-tivity/insecticidal activity/direct steam distillation/solvent extrac-tion-steam distillation/GC-MS引用本文复制引用
王国蕊,逄锦龙,张德蒙,李昉..龙须菜挥发油的提取及抗菌抗虫活性研究[J].食品与机械,2018,34(2):149-153,175,6.基金项目
海藻活性物质国家重点实验室开放基金项目(编号:SKL-BASS1717) （编号:SKL-BASS1717）
国家自然科学基金项目(编号:41106136) （编号:41106136）
山东省优秀中青年科学家科研奖励基金项目(编号:BS2012NY002) （编号:BS2012NY002）
青岛市科技计划基础研究项目(编号:1314144-JCH) （编号:1314144-JCH）
地方高校国家级大学生创新创业训练计划项目(编号:201602003) （编号:201602003）
