食品与机械2018，Vol.34Issue(2)：166-170,194,6.DOI:10.13652/j.issn.1003-5788.2018.02.035
大孔树脂纯化乌饭树叶黄酮工艺研究
Purification technology of flavones from Vaccinium Bracteatum Thunb leaves using macroporous resin
摘要
Abstract
In order to purify flavones from Vaccinium Bracteatum Thunb Leaves(VBTL)preliminarily,the static adsorption and de-sorption properties of four resins were compared.The purification technology of flavones from VBTL was investigated by dynamic ad-sorption and desorption experiments.The effect of sample concentra-tion,loading sample volume,ethanol solution for desorption,flow rate of the eluent and volume of eluent on adsorption and desorption rate were studied.In addition,the purification effect was evaluated by removal rate of proteins and polysaccharides and HPLC.The re-sults showed that NKA-II resin had a relatively high rate of adsorp-tion and desorption as well as a shorter adsorption time.NKA-II resin was determined as chromatographic column filler.The optimal purification technology was determined as follows:0.75 mg/mL for sample concentration,2 bed volume (BV)for loading sample volume,50% (v︰v)ethanol solution for desorption,1 mL/min for the flow rate of the eluent and 3 BV for the volume of eluent.HPLC anal-ysis of flavones from VBTL before and after purifying suggested the purity of purified flavones from VBTL improved significantly,and the re-moval rate of proteins and polysaccharides reached 76.32% and 65.45%respectively,with the purity of flavones reaching 48.92%.关键词
乌饭树/黄酮/纯化/大孔树脂Key words
Vaccinium Bracteatum Thunb/flavones/purification/macroporous resin引用本文复制引用
张德谨,陈义勇,胡雅琳,刘祥..大孔树脂纯化乌饭树叶黄酮工艺研究[J].食品与机械,2018,34(2):166-170,194,6.基金项目
宿州学院科研平台开放课题资助项目(编号:2016ykf03) （编号:2016ykf03）
宿州学院重点科研项目(编号:2016yzd03) （编号:2016yzd03）
安徽省大学生创新创业训练项目(编号:201610379120) （编号:201610379120）
