食品与机械2018，Vol.34Issue(2)：171-175,5.DOI:10.13652/j.issn.1003-5788.2018.02.036
野菊茎叶多糖微波辅助法提取工艺及抗氧化活性研究
Study on microwave-assisted extraction process for polysaccharide from the branches and leaves in Chrysanthemum indicum and its anti-oxidant activity
摘要
Abstract
The polysaccharide obtained from the branches and leaves of Chrysanthemum indicum was extracted by microwave assisted ex-traction after its powders decolorized and degreased,and then the polysaccharide extraction content was determined by phenol-sulfuric acid method.The extraction process of the polysaccharide was studied by single factor experiment which controlled liquid-solid ratio,extraction temperature,extraction time,and microwave power,and the orthogonal experiment was tested and based on single factor experiment,then determined the best extraction process. Those free radical cleared effects of its polysaccharide were tested by Fenton reaction and DPPH reaction.The results showed that the pol-ysaccharide extraction content from the branches and leaves of C.in-dicum could reach 6.32% when solid-liquid ratio was 1︰40 (g/mL), extracted by using microwave power (450 W)at 80 ℃ for 10 min. The anti-oxidant activity tests of the polysaccharide from the branches and leaves of C.indicum showed a good scavenging activity on ·OH and DPPH·,and the relationship between the polysaccha-ride and hydroxyl free radical clearance was found to be an concentra-tion reliable.Finally,the polysaccharide was verified to be an un-tapped antioxidant substance.关键词
野菊/茎叶/多糖/提取/抗氧化活性Key words
Chrysanthemum indicum/branches and leaves/polysac-charide/extraction/antioxidant activity引用本文复制引用
刘存芳,吴睿,赵桦,田光辉..野菊茎叶多糖微波辅助法提取工艺及抗氧化活性研究[J].食品与机械,2018,34(2):171-175,5.基金项目
陕西省教育厅服务地方专项项目(编号:15JF013) （编号:15JF013）
陕西省科技厅社会发展科技攻关项目(编号:2015SF270) （编号:2015SF270）
