中国食物与营养2018，Vol.24Issue(3)：27-29,3.
免疫亲和柱—超高效液相色谱法测定维生素饮料中维生素B12
The Determination of Vitamin B12 in Drinks with Immune Affinity Column-Ultra High Performance Liquid Chromatography
周黎 1余以刚 2徐红 1黄焯枝 2郭文婷 2郭伟鹏2
作者信息
- 1. 华南理工大学,广州510006
- 2. 达能(中国)食品饮料有限公司,广州510000
- 折叠
摘要
Abstract
Super efficient immune affinity column chromatography was adopted to establish the method for determination of the vitamin B12 in vitamin drink,sample after immune affinity column,purification,with 3 mL methanol elution at a speed of flow rate of 1 drop/See,nitrogen blow dry after dissolved by Ultra High Performance Liquid Chromatography (UPLC) method.Chromatographic column for HSS T3,column temperature 35 ℃,mobile phase gradient elution of acetonitrile-0.1％ formic acid solution,flow rate of 0.6 mL/min,the wavelength of 360 nm.Results showed that the concentration of vitamin B12 in the 0.4 ～ 10 μg/L range inside sex was good,the regression equation of y =4 040 x-4 040,the correlation coefficient was 0.997 00,recovery rate was 94.68％,the detection limit was 0.1 μg/L This method has the advantages of simple operation,high sensitivity,accurate,high detection efficiency,and is suitable for the determination of vitamin B12 in drinks with complex composition and low vitamin content.关键词
维生素B12/维生素饮料/免疫亲和柱/超高效液相色谱Key words
vitamin B12/vitamin drink/immune affinity column/Ultra High Performance Liquid Chromatography (UPLC)引用本文复制引用
周黎,余以刚,徐红,黄焯枝,郭文婷,郭伟鹏..免疫亲和柱—超高效液相色谱法测定维生素饮料中维生素B12[J].中国食物与营养,2018,24(3):27-29,3.
