食品工业科技2018，Vol.39Issue(7)：263-267,5.DOI:10.13386/j.issn1002-0306.2018.07.047
食品中单核细胞增生李斯特氏菌两种定量检测方法的比较
Comparison of two methods for detection of Listeria monocytogenes in foods
摘要
Abstract
Plate counting and MPN counting methods were used to detect Listeria monocytogenes in foods under differentconditions of contamination for screening an accurate one,and the quantity of LM in the refrigenerated and frozen foods atdifferent storage times was explored.The LM content of milk,salad and saltwater ducks with different degrees of artificialcontamination were detected by Plate counting and MPN counting methods respectively.The LM content of milk,salad andsaltwater ducks stored in cold storage(2～8 ℃)and frozen(-20 ℃)were tested by MPN counting method.The results showedthat the accuracy of Plate counting method was higher than MPN counting method when the concentration of bacteria was low(LM∶ Ecoli and SA is 10∶1)and the detection limit of LM was high(≥ 100 CFU/g(mL))while it was lower than MPNcounting method in the condition of the high initial concentration of bacteria(LM∶ Ecoli and SA is 1 ∶ 10) and the detection limitof LM(＜ 100 CFU/g(mL)).The number of LM in foods(milk,salad and saltwater ducks) increased with the increase of coldstorage and frozen time,and the number of LM in the above storage time of milk,salad and saltwater ducks had statisticsignificance(p ＜ 0.05).关键词
食品/平板计数法/MPN计数法/单核细胞增生李斯特氏菌Key words
foods/plate counting method/MPN counting method/Listeria monocytogenes分类
轻工纺织引用本文复制引用
马慧娟,徐慧,李俐俐,刘姝,贾涛,李新丽,高宏,张厚森..食品中单核细胞增生李斯特氏菌两种定量检测方法的比较[J].食品工业科技,2018,39(7):263-267,5.基金项目
江苏省科技社会发展项目(BE2016779). （BE2016779）
