食品工业科技2018，Vol.39Issue(8)：39-43,5.DOI:10.13386/j.issn1002-0306.2018.08.008
白茶乙醇提取物体外抗氧化活性研究
In vitro antioxidant capability of white tea ethanol extracts
摘要
Abstract
To investigate the antioxidant capability and cellular protective effect of Fuding white tea ethanol extracts (WTEE).Total tea polyphenols and flavonoids in WTEE were determinedby chemical colorimetry assay.The antioxidant capability of WTEE was evaluated according to the 1,1-diphenyl-2-picrylhydrazyl (DPPH)scavenging assay,superoxide anion scavenging assay and reducing power assay,respectively.Cellular protective effect of WTEE was investigated using a model of hydrogenperoxide (H2O2,150μmol/L)induced oxidativestress in intestinal epithelial Caco-2 cells.Cell viability was determined by MTT assay.The cellular levels of superoxide dismutase (SOD),and glutathione peroxidase (GSH-Px) were determined by commercial assay kits according the manuscripts.The cellular levels of malondiadehycle(MDA) were determined by thiobarbituric acid reactive substance (TBARS) assay.WTEE were enriched in total tea polyphenols (343.00 ± 27.90 mg gallic acid/g)and flavonoids(24.95 ±0.56 mg rutin/g).WTEE exhibited a great DPPH (ES50 330.63 μg/mL)and superoxide anion scavenging activity (EC50 342.90 μg/mL) and total reducing power.In addition,WTEE was able to increase the cell viability (to 85.6％)in oxidative damaged Caco-2 cells exposed to H2O2.The WTEE treatment also increased the cellular levels of SOD and GSH-Px,and reduced the oxidative damaged related MDA generation in H2O2 treated Caco-2 cells.Overall,the great in vitro antioxidant capability and cellular protective effect of WTEE was associated with the high levels of total tea polyphenols and flavonoids.关键词
白茶乙醇提取物/自由基清除能力/总还原力/Caco-2细胞/抗氧化活性Key words
white tea ethanol extracts/free radical scavenging activity/total reducing power/Caco-2 cells/antioxidant activity分类
轻工纺织引用本文复制引用
钱波,廖衫,刘斯悦,潘彩琳,何仲文,宋家乐..白茶乙醇提取物体外抗氧化活性研究[J].食品工业科技,2018,39(8):39-43,5.基金项目
桂林医学院引进人才科研启动基金(04010150001) （04010150001）
2017年教育部中西部高等学校青年骨干教师国内访问学者计划资助. （）
