食品工业科技2018，Vol.39Issue(8)：126-129,140,5.DOI:10.13386/j.issn1002-0306.2018.08.023
新型青霉素结合蛋白基因(Bt-pbp2)的克隆、表达及其在青霉素残留检测中的应用
The cloning and expression of novel penicillin binding protein (Bt-pbp2) and application in detection of penicillin residues
摘要
Abstract
The penicillin binding protein gene from bacillus thuringiesis (Bt-pbp2)was cloned and expressed in the host E.coli.Protein Bt-PBP2 extracted from E.coli was confirmed to have interaction with the penicillin G using the Microscale Thermophoresis(MST) technology.The equilibrium dissociation constant between Bt-PBP2 andpenicillin G (Kdvalue),was 10.36 nmol/L.The competitive enzyme-linked method of detection and determination ofpenicillin residues in Cow Milk was established according to the interaction between protein Bt-PBP2 and penicillin.Using the method,the dose-dependent linear relationshipis evident between the absorbance(A450) and the concentration of penicillin G in the range of 4～256 μg/L.The average adding recovery rate of this methodwas above 93％ ±5.24％.This research was showed that protein Bt-PBP2 is capable ofdetecting the penicillin residues in cow milk,and laid the foundationsfor the highly efficient test strip for the detection of penicillin residues.关键词
青霉素/青霉素结合蛋白/青霉素残留Key words
penicillin/penicillin binding protein/penicillin residues分类
轻工纺织引用本文复制引用
鞠守勇..新型青霉素结合蛋白基因(Bt-pbp2)的克隆、表达及其在青霉素残留检测中的应用[J].食品工业科技,2018,39(8):126-129,140,5.基金项目
湖北省j高等学校优秀中青年科技创新团队计划项目(T201535) （T201535）
武汉职业技术学院博士科研基金项目(2016BS005) （2016BS005）
湖北省教育厅科技B类项目(B2016583). （B2016583）
