食品工业科技2018，Vol.39Issue(8)：141-146,6.DOI:10.13386/j.issn1002-0306.2018.08.026
鼠尾藻岩藻黄素超声辅助提取及其纯化工艺优化
Optimization of ultrasonic assisted extraction and purification of fucoxanthin from Sargassum thunbergii
摘要
Abstract
Ultrasonic technology was used to assist the extraction of fucoxanthin from Sargassum thunbergii.The optimum conditions were determined by using single factor experiment and response surface methodology.The high performance liquid chromatography(HPLC) and electrospray ionization mass spectrometry (ESI/MS)method were developed for the analysis of fucoxanthin that was isolated and purified by silica gel column.The experimental results showed that the optimum ultrasonic extraction model was as follows:Ethanol concentration 80％ (v/v),ultrasonic starting temperature 52 ℃,extraction time 32 min,and solvent to material ratio 10 mL/g,the dosage of L-ascorbic acid 0.8％,extract two times,the fucoxanthin extraction yield was(0.455 ± 0.046) mg/g.Afier purification,the purity of the fucoxanthin isolated was about86.88％ ± 1.34％,and the extraction yield was(0.048 ±0.002) mg/g.This method that prepared the high purity fucoxanthin was simple and the extraction ratio was high,which would provide experimental basis and technical support for its comprehensive utilization in the future.关键词
鼠尾藻/岩藻黄素/超声辅助提取/纯化Key words
Sargassum thunbergii/fucoxanthin/ultrasonic assisted extraction/purification分类
轻工纺织引用本文复制引用
王乐,李丽,牟海津..鼠尾藻岩藻黄素超声辅助提取及其纯化工艺优化[J].食品工业科技,2018,39(8):141-146,6.基金项目
中央高校基本科研业务费(201762034) （201762034）
海洋公益性行业科研专项(201505022-5) （201505022-5）
山东省重点研发计划(2017YYSP003). （2017YYSP003）
