食品工业科技2018，Vol.39Issue(9)：11-15,22,6.DOI:10.13386/j.issn1002-0306.2018.09.003
超声预处理辅助酶解玉米胚芽ACE抑制肽的研究
Study on effects of ultrasonic pretreatment on enzymolysis preparation of ACE-inhibitory peptides from corn germ protein
摘要
Abstract
This study aimed to research the effects of ultrasonic pretreatment of different working modes on the preparation of ACE-inhibitory peptides from corn germ meal.According to protein conversion and proportion of highly active peptides,dualfrequency countercurrent energy-gathered ultrasound,triple-frequency divergence ultrasound and dual-frequency relative vibration ultrasound were used to pretreat corn germ meal and the best ultrasonic mode was selected.The ultrasonic preprocessing parameters were determined by single-factor step optimization method.Under ideal ultrasonic pretreatment conditions,enzymatic reaction conditions were optimized.Results showed that the ACE inhabitary activity of peptides of 300～ 1000 Da was the highest,IC50 of which was 0.78 mg/mL;the most effective ultrasonic mode was 20/40 kHz alternate dualfrequency and the optimum parameters of the ultrasonic pretreatment were the power density of 100 W/L,the substrate concentration of 8％,ultrasonic time of 20 min,ultrasonic temperature of 30 ℃.Enzymolysis conditions were enzyme dosage of 2000 U/g protein,enzymolysis time of 2.5 h.Under the optimal conditions,the protein conversion rate was 85.00％,which was 16.42％ higher than 73.01％ in the control group (without ultrasound),and the proportion of highly active peptides was 29.63％,which was 13.96％ higher than 26.00％ in the control group.The results showed that dual-frequency ultrasound could effectively improve the yield and the ACE inhabitary activity of the product,which was beneficial to the preparation of ACE inhibitory peptides.关键词
玉米胚芽蛋白/超声预处理/酶解/ACE抑制肽Key words
corn germ protein/ultrasonic pretreatment/enzymolysis/ACE-inhibitory peptides分类
轻工纺织引用本文复制引用
王珂,马海乐,李景,熊建,刘潇..超声预处理辅助酶解玉米胚芽ACE抑制肽的研究[J].食品工业科技,2018,39(9):11-15,22,6.基金项目
国家863计划(2013AA102203) （2013AA102203）
国家863计划重点项目(2007AA100404). （2007AA100404）
