食品工业科技2018，Vol.39Issue(9)：88-95,8.DOI:10.13386/j.issn1002-0306.2018.09.016
高产几丁质酶菌株的诱变选育及发酵条件的优化
Yield improvement in a chitinase-producing strain by mutation breeding and optimization of fermentation conditions
摘要
Abstract
A mutant strain GF-21 with high yield and stable inheritance was obtained by double round mutagenesis of ultraviolet -LiCl and microwave-LiCl using Serratia marcescens G3-1 as original strain.The fermentation conditions of GF-21 were investigated using single factor and orthogonal experiment.The result showed that the optimal fermentation medium contained 6 g/L lactose,10 g/L urea,1.0 mmol/L KCl and 5 g/L NaCl,10 g/L colloidal chitin.Under the optimal fermentation medium condition,the chitinase activity was 4.73 U/mL which was 1.09-fold higher than that before optimization and was 4.7-fold higher than original strain.The optimal fermentation conditions were as follows:the initial pH9.0,the temperature 30 ℃,the shaking speed 220 r/min,the inoculation age 5 h,the inoculum size 10％.The result provided a theoretical foundation for chitinase applications.关键词
几丁质酶/诱变/培养基优化/发酵条件优化Key words
chitinase/mutagenesis/medium optimization/fermentation optimization分类
轻工纺织引用本文复制引用
郑家敏,梁燕辉,朱凡,叶秀云,林娟..高产几丁质酶菌株的诱变选育及发酵条件的优化[J].食品工业科技,2018,39(9):88-95,8.基金项目
海洋经济创新发展区域示范项目(2014FJPT02) （2014FJPT02）
福建省中青年教师教育科研项目(JAT160054). （JAT160054）
