食品工业科技2018，Vol.39Issue(9)：96-101,6.DOI:10.13386/j.issn1002-0306.2018.09.017
高浓度培养乳酸菌发酵培养基的优化
Optimization of fermentation medium of lactic acid bacteria cultured in high concentration
摘要
Abstract
The optimization of fermentation medium for a strain of Lactobacillus R8,which had been proved to be resistant to acid and bile,lays a foundation for the probiotics to be used to improve the gastrointestinal tract healthy food.The components of fermentation medium were optimized by single factor test and orthogonal design test based on the density of bacteria.The results showed that the best fermentation formula for the bacterium was:glucose 20.0 g,yeast powder 30.0 g,MnSO4 ·4H2 O 0.075 g,MgSO4 ·7H2O 2.0 g,KH2PO4 2.5 g,citric acid ammonium 2.5 g,CH3COONa· 3H2O 6.25 g,Tween80 1.0 mL,pH6.2.After optimization,the concentration of bacteria(OD600 nm) reached 2.38.关键词
乳酸菌/益生菌/发酵培养基Key words
Lactic acid bacteria/probiotics/fermentation medium分类
轻工纺织引用本文复制引用
吴军林,柏建玲,莫树平,张菊梅..高浓度培养乳酸菌发酵培养基的优化[J].食品工业科技,2018,39(9):96-101,6.基金项目
广东省省级科技计划项目(2015A030302054) （2015A030302054）
广东省中国科学院全面战略合作专项(2013B091500024) （2013B091500024）
广东省自然科学基金(2014A030313667) （2014A030313667）
广州市科技项目(201508020068) （201508020068）
广东省科技项目(2016A010105012). （2016A010105012）
