食品工业科技2018，Vol.39Issue(9)：106-110,5.DOI:10.13386/j.issn1002-0306.2018.09.019
具有α-淀粉酶分泌活性而低产氨基甲酸乙酯的酵母菌构建
Construction of Saccharomyces cerevisiae with secretive a-amylase activity and low production of ethyl carbamate
摘要
Abstract
In order to construct Saccharomyces cerevisiae with α-amylase activity and low production of ethyl carbamate(EC).The expression vectors containing promotor sequence of ADH1 and ADH2 and α-amylase encoding sequence of Saccharomycopsis fibuligera were constructed,respectively.The α-amylase expression vectors were transformed into S△car1 and the positive recombinants were screened.The results indicated that the positive recombinants with α-amylase activity and reduced ethyl carbamate(EC) production were constructed and named S△car1/A1and S△car1/A2,respectively.In solid and liquid medium,α-amylase expression of S△car1/A1 was induced by glucose.Meanwhile,with the increasing of glucose concentration,the α-amylase expression was increased.However,the α-amylase of S△car1/A2 could be expressed without glucose and it could be feedback-inhibited by glucose.The results indicated α-amylase expression of S△car1/A2 was increased by low glucose concentration and reduced by high glucose concentration,although its expression was higher in the presence of glucose than without glucose.In conclusion,the ability of S△car1/A2 to produce α-amylase and hydrolyze starch was higher than S△car1/A1.In view of those,the S△car1/A2 could be used to ferment the materials containing starch.However,further research should be carried out about the condition of enzyme expression,catalyzing and fermentation.关键词
α-淀粉酶/酿酒酵母/氨基甲酸乙酯/碘熏蒸法/反馈抑制Key words
α-amylase/Saccharomyces cerevisiae/ethyl carbamate/iodine fumigation/feedback inhibition分类
轻工纺织引用本文复制引用
王佳,胡兰兰,陈福生,张秀艳..具有α-淀粉酶分泌活性而低产氨基甲酸乙酯的酵母菌构建[J].食品工业科技,2018,39(9):106-110,5.基金项目
中央高校基本科研业务费项目(2662015PY068) （2662015PY068）
国家自然科学基金资助(31071588) （31071588）
宁夏自治区重点研发计划重大项目(2016BZ06-03). （2016BZ06-03）
