食品工业科技2018，Vol.39Issue(10)：46-50,57,6.DOI:10.13386/j.issn1002-0306.2018.10.009
大豆11S球蛋白热力学特性和溶液性质与表面疏水性关系研究
Study on relationship between the thermodynamic characters/solution qroperties and surface hydrophobicity of soybean β-Conglycinin (11S)
摘要
Abstract
Taking β-Conglycinin (11 S)which was prepared with six representative soybean varieties as the object,Study the relationship between the thermodynamic characters/solution properties and surface hydrophobicity.Surface hydrophobicity of 11S was determined by ANS fluorescent probe method,thermodynamic characteristics was analyzed by differential scanning calorimetyt,molecular weight distribution was determined by size exclusion gel chromatography,hydrodynamic radius was analyzed by dynamic light scattering analyzer and ξ-potential was determined by Zeta-potential meter.The results showed that there was significantly negative correlation between surface hydrophobicity of 11S and thermal denaturation temperature (p =0.010)/denaturation enthalpy (p =0.012),and negatively correlated with solution properties (p =0.003).There was positive correlation between surface hydrophobicity of 11 S and average molecular weight (p =0.005)/average diameter(p =0.007),and negative correlation with ξ-potential absolute value(p =0.034).关键词
大豆11S球蛋白/热力学特性/分子量分布/流体动力学半径/ξ-电位/表面疏水性Key words
β-Conglycinin (11S)/thermodynamic characteristics/molecular weight distribution/hydrodynamic radius/ξ-potential/surface hydrophobicity分类
轻工纺织引用本文复制引用
李丹,魏冬旭,贾烨,刘春雷,江连洲..大豆11S球蛋白热力学特性和溶液性质与表面疏水性关系研究[J].食品工业科技,2018,39(10):46-50,57,6.基金项目
国家自然科学基金项目(C200101) （C200101）
福建省科技厅重点项目(2014N0007) （2014N0007）
宁德师范学院校级课题(2016Z07、2016Z08). （2016Z07、2016Z08）
