食品工业科技2018，Vol.39Issue(10)：110-116,7.DOI:10.13386/j.issn1002-0306.2018.10.021
烟曲霉素发酵培养基的优化
Optimization of fermentation medium for fumagillin production
摘要
Abstract
Objective:Medium was optimized for the production of fumagillin from Aspergillus fumigatus CEA-1701.Method:The content of fumagillin was determined by HPLC.The carbon and nitrogen sources were optimized using single factor experiment dessign.The major components in the medium were further optimized by Plackett-Burman (PB)and Box-Behnken Design (BBD).Results:Results show that the suitable sources of carbon and nitrogen for fumagillin productions are glycerol and yeast extract respectively;Glycerol,corn flour and yeast extract are the main factors affecting the production of fumagillin.BBD test showed that the optimum medium was composed of glycerol 33.5 g/L,yeast extract powder 3.1 g/L,corn flour 1.7 g/L,MgSO4·7H2 O 0.5 g/L,K2HPO4 1.5 g/L,KC1 0.5· g/L,FeSO4·7H2 O 0.013 g/L,which resulted in the fumagillin productivity of 68.51 mg/L that was 122.4％ higher than that of the initial medium.Conclusion:After the medium optimizition,the fumagillin production significantly increased,which provides a reference for fumagillin production.关键词
烟曲霉/烟曲霉素/培养基优化/碳源/氮源/响应面法Key words
Aspergillus fumigatus/fumagillin/medium optimization/crbon source/nitrogen source/pesponse surface methodology分类
轻工纺织引用本文复制引用
王昊鹏,吴黎明,赵柳微,倪辉,肖安风,张中印..烟曲霉素发酵培养基的优化[J].食品工业科技,2018,39(10):110-116,7.基金项目
北京市自然科学基金(6164044) （6164044）
国家现代农业蜂产业技术体系建设专项资金(CARS-45). （CARS-45）
