食品工业科技2018，Vol.39Issue(10)：122-127,6.DOI:10.13386/j.issn1002-0306.2018.10.023
植物生长调节剂促进蛹虫草液体表面培养生产虫草素
Plant growth regulators promote cordycepin production by liquid surface fermentation of Cordyceps militaris
摘要
Abstract
To further improve the cordycepin production,the effects of three plant growth regulators,triacontanol,gibberellin and 6-benzylaminopurine,and their combinations on mycelial growth of Cordyceps militaris and cordycepin accumulation were investigated in the surface liquid fermentation.Through central composite design and response surface method,a mathematical model was developed to identify the optimal value of plant growth regulators combination for high cordycepin production.The results showed that the three plant growth regulators can promote the growth of Cordyceps militaris and cordycepin accumulation to different degrees.When the three were used respectively,gibberellin exhibited the best growth promoting effect and the optimum concentration was 10 mg/L The triacontanol exhibited the best cordycepin-producing effect and the optimum addition was 0.6 mg/L.The optimal combination of 0.55 mg/L triacontanol,22.64 mg/L gibberellin and 1.69 mg/L 6-benzylaminopurine was the most favorable to cordycepin accumulation.Under the optimal conditions,the maximum cordycepin yield reached 7.31 g/L.Therefore,the combination of the plant growth regulators was favourable for cordycepin accumulation.关键词
三十烷醇/赤霉素/6-苄基腺嘌呤/蛹虫草/液体表面培养/虫草素Key words
triacontanol/gibberellin/6-benzylaminopurine/Cordyceps militaris/liquid surface culture/cordycepin分类
轻工纺织引用本文复制引用
汤佳鹏,汪建雄..植物生长调节剂促进蛹虫草液体表面培养生产虫草素[J].食品工业科技,2018,39(10):122-127,6.基金项目
江苏省普通高校自然科学研究计划项目(16KJB530004) （16KJB530004）
南通市应用基础研究项目(MS12015066). （MS12015066）
