食品工业科技2018，Vol.39Issue(10)：128-132,137,6.DOI:10.13386/j.issn1002-0306.2018.10.024
耙齿菌EA-LJS-73产七叶皂苷培养基配方的响应面法优化
Optimization of medium for producing escin Irpex sp.EA-LJS-73 by using response surface methodology
摘要
Abstract
Based on the single factor experiments,response surface methodology was employed to design,optimize and verify the culture medium for a endophytic fungus producing escin which was numbered as Irpex sp.EA-LJS-73,so as to improve the yield of escin.The results of optimum medium were as follows:starch 22.660 g,yeast extract 4.230 g,and MgSO4 7.600 g.Under these conditions,the predicted values of escin concentration could reach 1.116 g/L,the average concentration of the verification test was 1.105 g/L,which was 35％ higher than before.Through the analysis of fermentation kinetics,when the strain fermented 10 d,both the biomass and the concentration of escin were the highest,which were 11.376 g(dry weight of the mycelium)and 1.665 g/L,respectively.Therefore,the optimized medium was reasonable and feasible.关键词
耙齿菌/七叶皂苷/响应面分析/培养基优化/发酵特性Key words
Irpex sp./escin/response surface analysis/optimization of culture medium/fermentable characteristics分类
轻工纺织引用本文复制引用
刘军生,罗阳兰,解修超,邓百万,王娇,杨玉梅..耙齿菌EA-LJS-73产七叶皂苷培养基配方的响应面法优化[J].食品工业科技,2018,39(10):128-132,137,6.基金项目
秦巴山区生物资源综合开发协同创新中心自然科学研究项目[QBXT-Z(P)-15-2] （P）
陕西省教育厅科研专项项目(16JK1158). （16JK1158）
