食品工业科技2018，Vol.39Issue(10)：231-235,334,6.DOI:10.13386/j.issn1002-0306.2018.10.042
水解工艺对苦荞提取物中槲皮素含量的影响及其药代动力学研究
Effect of hydrolysis process on quercetin content in Tartary buckwheat extract and its pharmacokinetics
摘要
Abstract
Objective:To investigate the effect of hydrolysis process on quercetin content in Tartary Buckwheat extract and explore the acid hydrolysis influence on absorption of rutin and quercetin.Methods:Hydrochloric acid percentage,hydrolysis time,ethanol percentage and hydrolysis temperature were used to design a four-factor and three-level orthogonal test to optimize the acid hydrolysis process of rutin to quercertin,and hydrolyzate samples 1,2,3,4 with different quercetin contents were prepared at 1,2,4,6 h by this process.After oral administration of tartary buckwheat extract and hydrolysis samples (200 mg/kg),the blood samples were collected at the given time points.The concentration of quercetin was determined by HPLC,and the main pharmacokinetic parameters were calculated by DAS 2.0 software package.Results:The optimum parameters of rutin acid hydrolysis:The percentage of hydrochloric acid content,hydrolysis time,ethanol concentration and hydrolysis temperature were 0.91％,6 h,50％ and 70 ℃,respectively.And 64.11％ of rutin was hydrolyzed into quercetin under this process conditions.The AUC0-t of Tartary buckwheat extract,hydrolyzate samples 1,2,3and 4 equaled (17.604 ±5.422),(42.175 ± 9.435),(87.917 ± 19.347),(116.706 ± 46.256) and (178.509 ± 49.478) mg/L· h,respectively,while the C max of each sample was (2.743 ± 1.217),(7.109 ± 2.603),(12.438 ± 3.197),(15.727 ± 4.68) and (20.044 ± 5.736) mg/L,respectively.Compared to the Tartaryu buckwheat extract,the AUC0-t and Cmax of hydrolyzate samples 1,2,3 and 4 was significantly increased(p ＜ 0.05).Conclusions:With part of rutin convened to quercetin after acid hydrolysis,their cumulative absorption in rats was improved correspondingly.关键词
苦荞提取物/酸水解/药代动力学/累计吸收量Key words
Tartary buckwheat extract/acid hydrolysis/pharmacokinetics/cumulative absorption分类
轻工纺织引用本文复制引用
郑瑾,卓虹伊,宋雨,代丽萍,邓园源,邹亮,陈慧娟..水解工艺对苦荞提取物中槲皮素含量的影响及其药代动力学研究[J].食品工业科技,2018,39(10):231-235,334,6.基金项目
四川省科技计划项目(2015JY0259) （2015JY0259）
四川省教育厅高校科研创新团队(17TD0010). （17TD0010）
