食品工业科技2018，Vol.39Issue(10)：246-250,5.DOI:10.13386/j.issn1002-0306.2018.10.045
高效液相色谱法同时测定酵素原液中的乳酸和醋酸
Simultaneous determination of lactic acid and acetic acid in enzyme solution by high performance liquid chromatography
摘要
Abstract
A method for simultaneous determination of lactic acid and acetic acid in jiaosu solution by high performance liquid chromatography(HPLC) had been established.This article focused on the establishment of the simultaneous determination method of lactic acid and acetic acid in jiaosu solution by high performance liquid chromatography through the optimization of the detection wavelength,mobile phase and its concentration and pH.The results showed that the optimal Chromatographic conditions are as follows:Chromatographic column (SinoChrom ODS-BP 4.6 mm × 150 mm,5 μm),0.01 mol/L KH2PO4 solution(pH2.88) as mobile phase,flow rate of 0.8 mL/min,wavelength of 215 nm,injection volume of 20 μL,column temperature of 25 ℃.The method could be used for simultaneous determination of lactic acid and acetic acid in jiaosu solutions.The method has the advantages of fast detection,wide linear detection range of lactic acid (0～2100 μtg/mL) and acetic acid(0～1800 μg/mL),good precision and repeatability(the RSD of parallel determination of lactic acid and acetic acid were 0.99 ％ and 1.21％,respectively) and good recoveries (92.31％ ～ 102.20 ％).关键词
高效液相色谱法/酵素/乳酸/醋酸Key words
high performance liquid chromatography (HPLC)/jiaosu/lactic acid/acetic acid分类
轻工纺织引用本文复制引用
杨钰昆,宋佳,乔沈,郭园园,常媛媛,王兴华..高效液相色谱法同时测定酵素原液中的乳酸和醋酸[J].食品工业科技,2018,39(10):246-250,5.基金项目
国家自然科学基金青年科学基金项目(31701686) （31701686）
山西省应用基础研究项目面上青年基金项目(201701D221180) （201701D221180）
山西省科技厅项目(20140311021-5) （20140311021-5）
