食品工业科技2018，Vol.39Issue(10)：251-254,261,5.DOI:10.13386/j.issn1002-0306.2018.10.046
肉制品中牛源性成分Taqman荧光定量PCR检测法的建立
Establishment of Taqman fluorescence quantitative PCR assay for bovine derived ingredients in meat products
摘要
Abstract
In this experiment,specific PCR primers and Taqman probes were designed based on the differential mitochondrial DNA sequences of bovine mitochondria,and based on the 16S rDNA internal reference gene,universal primers and Taqman probes were designed.The total DNA concentration and DNA concentration of bovine source samples were determined by standard curve.Relative quantification method was used to determine the quality fraction of bovine source in meat products.The method was validated by specificity,sensitivity experiments,mixed meat recovery,and commercial meat testing.The results show that that method is specific and can amplify specific DNA of beef.The minimum observable limit with high sensitivity is 10 pg/μL.According to the recovery test,the average recovery was 98.62％,which can provide technical reference for the determination of beef content in meat products.关键词
Taqman探针/相对定量/牛源性/肉制品Key words
Taqman probe/relative quantitative/bovine origin/meat products分类
轻工纺织引用本文复制引用
李亮,赵新,刘娜,尚宏丽,王永,兰青阔,柏韵,王宁..肉制品中牛源性成分Taqman荧光定量PCR检测法的建立[J].食品工业科技,2018,39(10):251-254,261,5.基金项目
辽宁省自然科学基金项目(2015020776) （2015020776）
辽宁省农业领域创新人才项目(2014037) （2014037）
国家自然科学基金(81703636). （81703636）
