食品工业科技2018，Vol.39Issue(10)：308-314,7.DOI:10.13386/j.issn1002-0306.2018.10.057
干酪乳杆菌SY13及其合生元对小鼠肠道中Akkermansia muciniphila丰度的影响
Effects of Lactobacillus casei SY13 and its symbiotic on the abundance of Akkermansia muciniphila in mice intestine tract
摘要
Abstract
The abundance of Akkermansia muciniphila in the intestine was negatively correlated to a series of metabolic diseases such as obesity and type Ⅱ diabetes.In order to observe the effects of Lactobacillus casei SY13,SY13 and fructo-oligosaccharide,SY13 and lactulose on Akkermansia muciniphila in mice,8-week-old mice and 9-month-old mice were studied.The mice were randomly divided into 5 groups:AP group (8-week-old mice,gavaged for 1 day,once a day),AO group (9-months-old mice,gavage for 1 day,once a day),BP group (8-week-old mice,gavage 28 days,once a day),BO group (9-month-old mice,gavage 28 days,once a day),48 in each group.Each experimental group was divided into control group (sterile PBS,0.3 mL),Lactobacillus casei SY13 group(SY13,109 cfu,0.3 mL),SY13 fructo-oligosaccharide group(109 cfu;Fructooligosaccharides,15 mg;0.3 mL)and SY13 lactulose group (SY13:109 cfu,lactulose:15 mg,0.3 mL),12 ineach group.The abundance of Akkermansia muciniphila in jejunum,ileum,cecum and colon of mice were detected by real-time fluorescence quantitative PCR.The abundance of Akkermansia muciniphila indirectly reflectedby CT values.The results showed that the CT value of SY13 lactulose group was significantly lower than that of the other groups(p ＜ 0.05),the CT value of young mice was significantly lower than that of the aged group(p ＜ 0.05).Compared with single gavage,CT value was lower in long-term gavage (p ＜0.05).The research showed that,SY13-lactulose group had the best effect on increasing the abundance of Akkermansia muciniphila in the intestine ofmice,and the lower the mice age,the longer the gavage time,the higher abundance of Akkermansia muciniphila.At the same time,the longer the gavage,the longer the retention time of Akkermansia muciniphila,which was not related to the age of mice and gavage.关键词
Akkermansia muciniphila/干酪乳杆菌SY13/合生元/实时荧光定量PCRKey words
Akkermansia muciniphila/Lactobacillus casei SY13/synbiotics/real-time fluorescence quantitative PCR分类
轻工纺织引用本文复制引用
提盼盼,汪建明,逄晓阳,吕加平..干酪乳杆菌SY13及其合生元对小鼠肠道中Akkermansia muciniphila丰度的影响[J].食品工业科技,2018,39(10):308-314,7.基金项目
国家自然科学基金面上项目(31471603) （31471603）
国家科技支撑计划项目(2015BAD16B00) （2015BAD16B00）
中国农业科学院科技创新工程项目(cxgc-ifst-08). （cxgc-ifst-08）
